IL-4I1/LAO Antibody (YA1225)(PBS only)

Cat. No.: HY-P81480A: Data Sheet User Guide for Antibodies
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IL-4I1/LAO Antibody (YA1225) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-4I1/LAO.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

IL-4I1/LAO Antibody (YA1225) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-4I1/LAO.

Host

Rabbit

Molecular Weight

Predicted band size: 63 kDa;

Observed band size: 75 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q96RQ9

Gene ID

259307 [NCBI]

Immunogen

Recombinant protein of human IL4I1

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Secreted L-amino-acid oxidase that acts as a key immunoregulator (PubMed:17356132, PubMed:32818467, PubMed:32866000). Has preference for L-aromatic amino acids: converts phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp) to phenylpyruvic acid (PP), hydroxyphenylpyruvic acid (HPP), and indole-3-pyruvic acid (I3P), respectively (PubMed:17356132, PubMed:32818467, PubMed:32866000). Also has weak L-arginine oxidase activity (PubMed:26673964). Acts as a negative regulator of anti-tumor immunity by mediating Trp degradation via an indole pyruvate pathway that activates the transcription factor AHR (PubMed:32818467, PubMed:32866000). IL4I1-mediated Trp catabolism generates I3P, giving rise to indole metabolites (indole-3-acetic acid (IAA) and indole-3-aldehyde (I3A)) and kynurenic acid, which act as ligands for AHR, a ligand-activated transcription factor that plays important roles in immunity and cancer (PubMed:32818467, PubMed:32866000). AHR activation by indoles following IL4I1-mediated Trp degradation enhances tumor progression by promoting cancer cell motility and suppressing adaptive immunity (PubMed:32818467). Also has an immunoregulatory function in some immune cells, probably by mediating Trp degradation and promoting downstream AHR activation: inhibits T-cell activation and proliferation, promotes the differentiation of naive CD4(+) T-cells into FOXP3(+) regulatory T-cells (Treg) and regulates the development and function of B-cells (PubMed:17356132, PubMed:25446972, PubMed:25778793, PubMed:28891065). Also regulates M2 macrophage polarization by inhibiting T-cell activation (By similarity). Also has antibacterial properties by inhibiting growth of Gram negative and Gram positive bacteria through the production of NH4(+) and H2O2 (PubMed:23355881)

Subcellular Localization：Secreted; Lysosome; Cytoplasmic vesicle, secretory vesicle, acrosome

Expression：
Tissue_specificity:Primarily found in immune tissues, with the highest expression in lymph nodes and spleen (PubMed:12031486, PubMed:12446450) . Present in germinal center macrophages and inflammatory myeloid cells and antigen-presenting cells (at protein level) (PubMed:17356132) . Also present in spermatozoa (at protein level) (PubMed:25767141) . Highly expressed in primary mediastinal large B-cell lymphoma, a specific subtype of diffuse large B-cell lymphoma (PubMed:12446450) . Expressed by neoplastic cells of several B-cell lymphomas and by tumor-associated macrophages (PubMed:19436310)

Induction:By IL4/interleukin-4 (PubMed:12031486) . Expression is up-regulated in numerous cancer and metastasis: expression is induced by immune checkpoint blockade (PubMed:32818467)

Isoforms & Post-Translational Modification：Q96RQ9 has 2 isomers: Q96RQ9-1: 62881 Da (predicted); Q96RQ9-2: 65328 Da (predicted).
N-glycosylated

Research Field

Cell Biology

Synonyms

IL4I1; FIG1 ; UNQ636/PRO1265 ; L-amino-acid oxidase ; Interleukin-4-induced protein 1; hIL4I1 ; Protein Fig-1 ; LAAO; LAO

Documentation

User Guide for Antibodies (1077 KB)