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Parathyroid Hormone Receptor 1 Antibody (YA4175)

(Synonyms: PFE; PTHR; PTHR1; MGC138426; MGC138452; PTH1R)
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Based on 1 Customer Validation

Parathyroid Hormone Receptor 1 Antibody (YA4175) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Parathyroid Hormone Receptor 1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Parathyroid Hormone Receptor 1 Antibody (YA4175)
  • Parathyroid Hormone Receptor 1 Antibody (YA4175) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:1000 1:10000

Product Details

Description

Parathyroid Hormone Receptor 1 Antibody (YA4175) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Parathyroid Hormone Receptor 1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 66 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 66 kDa
Immunogen

Purified recombinant fragment of human PTH1R aa 27-188.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Parathyroid Hormone Receptor 1 Antibody (YA4175)
    Immunofluorescence analysis of Hela cells labeling PTH1R antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with PTH1R antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

Background

  • Function

    Parathyroid Hormone Receptor 1 is a G-protein-coupled receptor for parathyroid hormone (PTH) and for parathyroid hormone-related peptide (PTHLH). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (cAMP). PTH1R is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity. PTHLH dissociates from PTH1R more rapidly than PTH; as consequence, the cAMP response induced by PTHLH decays faster than the response induced by PTH[1][2][3][4][5][6][7][8].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:It is expressed in most tissues, with the highest concentrations found in the kidneys, bones, and liver.

  • Subunit

    Homodimer in the absence of bound ligand (PubMed:19674967, PubMed:20172855, PubMed:27160269, PubMed:30975883). Peptide hormone binding leads to dissociation of the homodimer (PubMed:19674967, PubMed:20172855)

  • SwissProt ID

    Q03431

  • Gene ID
    5745 [NCBI]
  • Synonyms

    PFE; PTHR; PTHR1; MGC138426; MGC138452; PTH1R

Parathyroid Hormone Receptor 1 Antibody (YA4175) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling PTH1R antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with PTH1R antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • Parathyroid Hormone Receptor 1 Antibody (YA4175) Product Image
    1/16
  • Experimental Validation Results for Parathyroid Hormone Receptor 1 Antibody (YA4175)
  • Parathyroid Hormone Receptor 1 Antibody (YA4175) Product Image
  • Experimental Validation Results for Parathyroid Hormone Receptor 1 Antibody (YA4175)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling PTH1R antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with PTH1R antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)
  • Experimental Validation Results for Parathyroid Hormone Receptor 1 Antibody (YA4175)
  • Parathyroid Hormone Receptor 1 Antibody (YA4175) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of Hela cells labeling PTH1R antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with PTH1R antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001, green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • Parathyroid Hormone Receptor 1 Antibody (YA4175) Product Image ICC