RIC8A (RIC8) Antibody (YA7939)(PBS only)

Cat. No.: HY-P88255A: Data Sheet User Guide for Antibodies
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RIC8A (RIC8) Antibody (YA7939) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RIC8A (RIC8).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

RIC8A (RIC8) Antibody (YA7939) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RIC8A (RIC8).

Host

Mouse

Molecular Weight

Predicted band size: 59.6 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9NPQ8

Immunogen

Full length human recombinant protein of human RIC8A produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-250
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Chaperone that specifically binds and folds nascent G alpha proteins prior to G protein heterotrimer formation, promoting their stability and activity: folds GNAI1, GNAO1, GNA13 and GNAQ (By similarity). Does not fold G(s) G-alpha proteins GNAS nor GNAL (By similarity). Also acts as a guanine nucleotide exchange factor (GEF) for G alpha proteins by stimulating exchange of bound GDP for free GTP (By similarity). Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein (GNAI1), possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation (PubMed:16629901)

Subcellular Localization：Cytoplasm, cell cortex,Cytoplasm

Isoforms & Post-Translational Modification：Q9NPQ8 has four isomers: Q9NPQ8-1: 59710 Da (predicted); Q9NPQ8-2: 58943 Da (predicted); Q9NPQ8-3: 60372 Da (predicted); Q9NPQ8-4: 59612 Da (predicted).
Phosphorylated at Ser-436 and Thr-441 by CK2, stabilizing its interface with G alpha proteins

Subunit：Interacts with GDP-bound G alpha proteins GNAI1, GNAO1 and GNAQ, and with GNA13 with lower affinity (By similarity)

Synonyms

RIC8

Documentation

User Guide for Antibodies (1077 KB)