Osteopontin Antibody (YA3743)
(Synonyms: OPN; BNSP; BSPI; ETA-1)Osteopontin Antibody (YA3743) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Osteopontin.
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Host:
Mouse
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Isotype:
IgG
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Application:
IHC-P, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|
| Dilution Ratio | 1:200-1:400 | 1:10000 |
Product Details
Osteopontin Antibody (YA3743) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Osteopontin.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 60 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 35 kDa
Purified recombinant fragment of human SPP1 (AA: 167-314) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Osteopontin (OPN) is a secreted, multifunctional, extracellular matrix (ECM), calcium-binding, glycosylated phosphoprotein that is associated with various normal biological functions and pathological processes. OPN is physiologically produced by a wide range of cells and has been identified in various body fluids, playing a crucial role in bone remodeling and vascularization. OPN is also expressed by multiple immune cells, acting as a chemotactic factor, a mediator of cell activation and cytokine production, and an anti-apoptotic factor, thereby regulating inflammation and immune responses. OPN can be involved in the tumorigenesis of various cancer types, including head and neck squamous cell carcinoma (HNSCC), by promoting cell proliferation, adhesion, invasion, and migration through multiple signaling pathways[1][2].
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Subcellular Localization
Secreted
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Expression
Tissue_specificity:Protein levels were detected in cerebrospinal fluid and urine (PubMed:25326458, PubMed:36213313, PubMed:37453717) . In bone. Found in plasma. -
Isoforms & Post-Translational Modification
P10451 has 4 isomers: P10451-1: 35423 Da (predicted); P10451-3: 32355 Da (predicted); P10451-4: 33017 Da (predicted); P10451-5: 33843 Da (predicted).
Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties;Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif (PubMed:15869464, PubMed:22582013, PubMed:26091039). The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization (By similarity);O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62 -
Subunit
Interacts (via N-terminus) with integrin ITGA9:ITGB1
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SwissProt ID
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Synonyms
OPN; BNSP; BSPI; ETA-1
Documentation
References
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