UCP3 Antibody (YA4106)(PBS only)

Cat. No.: HY-P84409A: Data Sheet User Guide for Antibodies
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UCP3 Antibody (YA4106) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to UCP3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

UCP3 Antibody (YA4106) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to UCP3.

Host

Mouse

Molecular Weight

Predicted band size: 34 kDa;

Observed band size: 34 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P55916

Gene ID

7352 [NCBI]

Immunogen

Purified recombinant fragment of human UCP3 (AA: 1-113 and 217-312) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Putative transmembrane transporter that plays a role in mitochondrial metabolism via an as yet unclear mechanism (PubMed:21775425, PubMed:36114012). Originally, this mitochondrial protein was thought to act as a proton transmembrane transporter from the mitochondrial intermembrane space into the matrix, causing proton leaks through the inner mitochondrial membrane, thereby uncoupling mitochondrial membrane potential generation from ATP synthesis (PubMed:11171965, PubMed:12670931, PubMed:12734183, PubMed:9305858). However, this function is controversial and uncoupling may not be the function, or at least not the main function, but rather a consequence of more conventional metabolite transporter activity (PubMed:11707458)

Subcellular Localization：Mitochondrion inner membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Only in skeletal muscle and heart (PubMed:9305858) . Also expressed in white and brown adipose tissues (PubMed:9305858) . Is more expressed in glycolytic than in oxidative skeletal muscles

Induction:Up-regulated by beta3-adrenergic stimulation, starvation, glucocorticoids and leptin

Isoforms & Post-Translational Modification：P55916 has 3 isomers: P55916-1: 34216 Da (predicted); P55916-2: 29783 Da (predicted); P55916-3: 22874 Da (predicted).

Subunit：Interacts with HAX1; the interaction is direct and calcium-dependent

Synonyms

SLC25A9

Documentation

User Guide for Antibodies (1077 KB)