Cholic acid
Based on 21 publication(s) in Google Scholar
Cholic acid is a major primary bile acid produced in the liver and usually conjugated with glycine or taurine. It facilitates fat absorption and cholesterol excretion. Cholic acid is orally active.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.80%
- CAS. Nr.: 81-25-4
- Formel: C24H40O5
- Molecular Weight:408.57
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
Publications Citing Use of MedChemExpress (MCE) Cholic acid
More- Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8. [Abstract]
- Nature. 2025 Jul;643(8070):192-200. [Abstract]
- Cell Res. 2019 Mar;29(3):193-205. [Abstract]
- Adv Mater. 2025 Apr;37(17):e2416349. [Abstract]
- Cell Host Microbe. 2025 Aug 19:S1931-3128(25)00291-4. [Abstract]
- Cell Host Microbe. 2024 Feb 14;32(2):191-208.e9. [Abstract]
- J Adv Res. 2024 Sep 5:S2090-1232(24)00389-8. [Abstract]
- Trends Biotechnol. 2026 Apr 15:S0167-7799(26)00130-7. [Abstract]
- Adv Sci (Weinh). 2025 Feb 3:e2411719. [Abstract]
- Research (Wash D C). 2025 Jun 24:8:0741. [Abstract]
- J Transl Med. 2024 Dec 20;22(1):1124. [Abstract]
- Cell Rep. 2025 Sep 17;44(10):116306. [Abstract]
- Mol Neurobiol. 2024 Nov;61(11):9302-9319. [Abstract]
- J Enzyme Inhib Med Chem. 2025 Dec;40(1):2501743. [Abstract]
- Front Cell Dev Biol. 2022 Jul 22:10:854425. [Abstract]
- Aquaculture. 2023 Sep 18, 740123.
- Microb Pathog. 2026 Jan:210:108187. [Abstract]
- Drug Metab Dispos. 2026 Feb 24.
- J Pharm Biomed Anal. 2025 Jul 15:259:116760. [Abstract]
- J Voice. 2025 Nov 29:S0892-1997(25)00490-4. [Abstract]
- Transl Cancer Res. 2025 Aug 31;14(8):4774-4790. [Abstract]
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RT-PCR
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In Vivo Efficacy Study
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Bio/Physico-chemical Assay
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RT-PCR
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Histological Imaging/Staining
Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen
More
Biologische Aktivität
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Human Endogenous Metabolite |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
>200 μM
Compound: CA
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Anticancer activity against human A549 cells assessed as inhibition of cell proliferation by MTT assay
Anticancer activity against human A549 cells assessed as inhibition of cell proliferation by MTT assay
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[PMID: 36439975] |
| CHO | EC50 |
13.6 μM
Compound: 3, CA
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Agonist activity at human TGR5 expressed in CHO cells after 5 hrs by CRE-driven luciferase reporter gene assay
Agonist activity at human TGR5 expressed in CHO cells after 5 hrs by CRE-driven luciferase reporter gene assay
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[PMID: 17685603] |
| CHO | EC50 |
13.6 μM
Compound: 5, CA
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Agonist activity at human TGR5 expressed in CHO cells by luciferase assay
Agonist activity at human TGR5 expressed in CHO cells by luciferase assay
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[PMID: 18307294] |
| CHO | EC50 |
13.6 μM
Compound: 4, CA
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Agonist activity at human TGR5 expressed in CHO cells assessed as increase in CRE-driven gene expression by luciferase reporter gene assay
Agonist activity at human TGR5 expressed in CHO cells assessed as increase in CRE-driven gene expression by luciferase reporter gene assay
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[PMID: 20014870] |
| COS-1 | EC50 |
>100 μM
Compound: 4, CA
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Agonist activity at FXR expressed in COS1 cells by cell-based bioluminescence assay
Agonist activity at FXR expressed in COS1 cells by cell-based bioluminescence assay
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[PMID: 20014870] |
| GBM | IC50 |
>50 μM
Compound: 1a, CA, cholic acid
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Cytotoxicity against human GBM cells after 24 hrs by neutral red uptake assay
Cytotoxicity against human GBM cells after 24 hrs by neutral red uptake assay
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[PMID: 20381215] |
| HCT-116 | IC50 |
>50 μM
Compound: 1a, CA, cholic acid
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Cytotoxicity against human HCT116 cells after 24 hrs by neutral red uptake assay
Cytotoxicity against human HCT116 cells after 24 hrs by neutral red uptake assay
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[PMID: 20381215] |
| HCT-116 | IC50 |
>200 μM
Compound: CA
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Anticancer activity against human HCT-116 cells assessed as inhibition of cell proliferation by MTT assay
Anticancer activity against human HCT-116 cells assessed as inhibition of cell proliferation by MTT assay
|
[PMID: 36439975] |
| HEK293 | IC50 |
>1000 μM
Compound: 25
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Antiproliferative activity against HEK293 cells by MTT assay
Antiproliferative activity against HEK293 cells by MTT assay
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[PMID: 18805690] |
| HEK293 | IC50 |
5.3 μM
Compound: 1, CA
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Agonist activity at mouse TGR5 receptor expressed in HEK293 cells assessed as intracellular cAMP level
Agonist activity at mouse TGR5 receptor expressed in HEK293 cells assessed as intracellular cAMP level
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[PMID: 20801037] |
| HEK293 | IC50 |
6 μM
Compound: 1, CA
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Agonist activity at human TGR5 receptor expressed in HEK293 cells assessed as intracellular cAMP level
Agonist activity at human TGR5 receptor expressed in HEK293 cells assessed as intracellular cAMP level
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[PMID: 20801037] |
| HEK-293T | EC50 |
>150 μM
Compound: CA
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Agonist activity at VP16 tagged-VDR-LBD (unknown origin) expressed in HEK293T cells assessed as SRC1 coactivator peptide recruitment after 16 hrs by luciferase reporter gene based two hybrid assay
Agonist activity at VP16 tagged-VDR-LBD (unknown origin) expressed in HEK293T cells assessed as SRC1 coactivator peptide recruitment after 16 hrs by luciferase reporter gene based two hybrid assay
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[PMID: 26774929] |
| HEK-293T | IC50 |
>150 μM
Compound: CA
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Antagonist activity against VP16 tagged-VDR-LBD (unknown origin) expressed in HEK293T cells assessed as inhibition of 1,25-dihydroxyvitamin D3-induced SRC1 coactivator peptide recruitment after 16 hrs by luciferase reporter gene based two hybrid assay
Antagonist activity against VP16 tagged-VDR-LBD (unknown origin) expressed in HEK293T cells assessed as inhibition of 1,25-dihydroxyvitamin D3-induced SRC1 coactivator peptide recruitment after 16 hrs by luciferase reporter gene based two hybrid assay
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[PMID: 26774929] |
| HET-1A | CC50 |
1075 μM
Compound: 4, CA
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Cytotoxicity against human HET-1A cells assessed as cell viability after 24 hrs by MTT assay
Cytotoxicity against human HET-1A cells assessed as cell viability after 24 hrs by MTT assay
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[PMID: 20713311] |
| HT-29 | IC50 |
>200 μM
Compound: CA
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Anticancer activity against human HT-29 cells assessed as inhibition of cell proliferation by MTT assay
Anticancer activity against human HT-29 cells assessed as inhibition of cell proliferation by MTT assay
|
[PMID: 36439975] |
| Huh-7 | IC50 |
>200 μM
Compound: CA
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Anticancer activity against human Huh-7 cells assessed as inhibition of cell proliferation by MTT assay
Anticancer activity against human Huh-7 cells assessed as inhibition of cell proliferation by MTT assay
|
[PMID: 36439975] |
| KMS-11 | IC50 |
>50 μM
Compound: 1a, CA, cholic acid
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Cytotoxicity against human KMS11 cells after 24 hrs by neutral red uptake assay
Cytotoxicity against human KMS11 cells after 24 hrs by neutral red uptake assay
|
[PMID: 20381215] |
| LoVo | IC50 |
>200 μM
Compound: CA
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Anticancer activity against human LoVo cells assessed as inhibition of cell proliferation by MTT assay
Anticancer activity against human LoVo cells assessed as inhibition of cell proliferation by MTT assay
|
[PMID: 36439975] |
| MCF7 | IC50 |
50 μM
Compound: 25
|
Antiproliferative activity against human MCF7 cells by MTT assay
Antiproliferative activity against human MCF7 cells by MTT assay
|
[PMID: 18805690] |
| MGC-803 | IC50 |
>200 μM
Compound: CA
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Anticancer activity against human MGC-803 cells assessed as inhibition of cell proliferation by MTT assay
Anticancer activity against human MGC-803 cells assessed as inhibition of cell proliferation by MTT assay
|
[PMID: 36439975] |
| NCI-H716 | EC50 |
13.6 μM
Compound: 1, CA
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Agonist activity at TGR5 expressed in NCI-H716 cells assessed as cAMP level after 60 mins by FRET analysis
Agonist activity at TGR5 expressed in NCI-H716 cells assessed as cAMP level after 60 mins by FRET analysis
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[PMID: 24900463] |
| RKO | IC50 |
>200 μM
Compound: CA
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Anticancer activity against human RKO cells assessed as inhibition of cell proliferation by MTT assay
Anticancer activity against human RKO cells assessed as inhibition of cell proliferation by MTT assay
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[PMID: 36439975] |
| SK-HEP1 | IC50 |
>200 μM
Compound: CA
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Anticancer activity against human SK-HEP1 cells assessed as inhibition of cell proliferation by MTT assay
Anticancer activity against human SK-HEP1 cells assessed as inhibition of cell proliferation by MTT assay
|
[PMID: 36439975] |
| SW480 | IC50 |
>200 μM
Compound: CA
|
Anticancer activity against human SW480 cells assessed as inhibition of cell proliferation by MTT assay
Anticancer activity against human SW480 cells assessed as inhibition of cell proliferation by MTT assay
|
[PMID: 36439975] |
Cholic acid (1 mg/mL, 30 min) competitively binds Na+/taurocholate cotransporting polypeptide (NTCP) on HepG2 cells and significantly inhibits the uptake of Cholic acid (CA)-nanoliposomes (LPs)-Doxorubicin (DOX)-HCl, which indicates that CA-LPs-DOX-HCl are also uptaken via NTCP-mediated endocytosis pathway[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Tg-CYP2D6 adult male mice (8 weeks of age and weighing 20–25 g)[2]
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Dosage:1% (w/w) Cholic acid-supplemented diet
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Administration:Oral, 14 days
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Result:Decreases SHP expression and increased CYP2D6 activity.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS. Nr. 81-25-4
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Appearance Solid
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Molecular Weight 408.57
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Formel C24H40O5
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Color White to off-white
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SMILES
C[C@H](CCC(O)=O)[C@@]1([H])CC[C@@]2([H])[C@]3([H])[C@H](O)C[C@]4([H])C[C@H](O)CC[C@]4(C)[C@@]3([H])C[C@H](O)[C@@]21C
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Structure Classification
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (21)
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Journal Impact Factor
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Most Recent
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Cancer Cell
Integrative plasma and fecal metabolomics identify functional metabolites in adenoma-colorectal cancer progression and as early diagnostic biomarkers. [Abstract]2024 Aug 12;42(8):1386-1400.e8. PMID: 39137727 -
Nature
2025 Jul;643(8070):192-200. PMID: 39695227 -
Cell Res
Virus-induced accumulation of intracellular bile acids activates the TGR5-β-arrestin-SRC axis to enable innate antiviral immunity. [Abstract]2019 Mar;29(3):193-205. PMID: 30651583 -
Adv Mater
2025 Apr;37(17):e2416349. PMID: 40025988 -
Cell Host Microbe
2025 Aug 19:S1931-3128(25)00291-4. PMID: 40848719 -
Cell Host Microbe
A gut microbiota-bile acid axis promotes intestinal homeostasis upon aspirin-mediated damage. [Abstract]2024 Feb 14;32(2):191-208.e9. PMID: 38237593 -
J Adv Res
Serum taurine affects lung cancer progression by regulating tumor immune escape mediated by the immune microenvironment. [Abstract]2024 Sep 5:S2090-1232(24)00389-8. PMID: 39243941
Cholic acid purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 Sep 5:S2090-1232(24)00389-8. [Abstract]
The tumor-promoting effects of taurine could be reversed by bile acids (Cholic acid (245.25 mg/kg; p.o.; Once daily)) in C57 mice (n = 6).
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Trends Biotechnol
Engineering Bacillus subtilis as a sustainable platform for the production of functional bile acids. [Abstract]2026 Apr 15:S0167-7799(26)00130-7. PMID: 41991462 -
Adv Sci (Weinh)
Targeting FDFT1 Reduces Cholesterol and Bile Acid Production and Delays Hepatocellular Carcinoma Progression Through the HNF4A/ALDOB/AKT1 Axis. [Abstract]2025 Feb 3:e2411719. PMID: 39899681
Cholic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb 3:e2411719. [Abstract]
The mRNA levels of ALDOB in Huh7 or HCCLM3 cells treated with various bile acids (100 μM, 24 h). All data are presented as mean ± SD. Data were analyzed by one-way ANOVA with Bonferroni multiple-comparison correction. CA, cholic acid; TCA, taurocholic acid; GCA, glycocholic acid; TCDCA, taurochenodeoxycholic acid; GCDCA, glycochenodeoxycholic acid; LCA, lithocholic acid; TLCA, taurolithocholic acid; GLCA, glycolithocholic acid; DCA, deoxycholic acid; TDCA, taurodeoxycholic acid; GDCA, glycodeoxycholic acid; UDCA, ursodeoxycholic acid; TUDCA, tauroursodeoxycholic acid; GUDCA, glycoursodeoxycholic acid.
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Research (Wash D C)
Single-Cell RNA Transcriptomics and Multi-omics Analyses Reveal the Clinical Effects of Acupuncture on Methadone Reduction. [Abstract]2025 Jun 24:8:0741. PMID: 40556944 -
J Transl Med
Conjugated bile acids alleviate acute pancreatitis through inhibition of TGR5 and NLRP3 mediated inflammation. [Abstract]2024 Dec 20;22(1):1124. PMID: 39707318 -
Cell Rep
Multilevel regulation of c-di-GMP biosynthesis by cAMP signaling increases Shigella sonnei fitness and pathogenicity in response to bile salts. [Abstract]2025 Sep 17;44(10):116306. PMID: 40966079 -
Mol Neurobiol
Brain Immune Cell Infiltration and Serum Metabolomic Characteristics Reveal that Lauric Acid Promotes Immune Cell Infiltration in Brain and Streptococcus suis Meningitis in Mice. [Abstract]2024 Nov;61(11):9302-9319. PMID: 38625620 -
J Enzyme Inhib Med Chem
Natural product sennoside B disrupts liquid-liquid phase separation of SARS-CoV-2 nucleocapsid protein by inhibiting its RNA-binding activity. [Abstract]2025 Dec;40(1):2501743. PMID: 40371698 -
Front Cell Dev Biol
Comprehensive Analysis of Quantitative Proteomics With DIA Mass Spectrometry and ceRNA Network in Intrahepatic Cholestasis of Pregnancy. [Abstract]2022 Jul 22:10:854425. PMID: 35938169
Cholic acid purchased from MedChemExpress. Usage Cited in: Front Cell Dev Biol. 2022 Jul 22:10:854425. [Abstract]
HE results of the placental explant. NC Group: normal placenta; ICP group: normal placenta was stimulated for 48 h with 100 uM Cholic acid.
Cholic acid purchased from MedChemExpress. Usage Cited in: Front Cell Dev Biol. 2022 Jul 22:10:854425. [Abstract]
Western blotting results and gray histogram of the placental explant. NC group: normal placenta; ICP group: the normal placenta was stimulated for 48 h with 100 uM Cholic acid.
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Cholic acid purchased from MedChemExpress. Usage Cited in: Aquaculture. 2023 Sep 18, 740123.
The results showed that dietary Cholic acid (CA, 100 mg/kg) supplementation increased the levels of T-CHO, LDL-C, and HDL-C (P < 0.05).
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Microb Pathog
Lithocholic acid exerts antiviral activity against porcine epidemic diarrhea virus by enhancing TGR5-mediated type III interferon production. [Abstract]2026 Jan:210:108187. PMID: 41242567 -
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J Pharm Biomed Anal
Integrating 16S rDNA sequencing analysis and targeted metabolomics to explore the mechanism of Xiexin Tang in treating atherosclerosis mice induced by high-fat diet. [Abstract]2025 Jul 15:259:116760. PMID: 40014894 -
J Voice
Deoxycholic Acid Drives ATP4A-Dependent Growth and Migration of Vocal Fold Leukoplakia Epithelial Cells via TGR5/JAK2/STAT3 Signaling. [Abstract]2025 Nov 29:S0892-1997(25)00490-4. PMID: 41320587 -
Transl Cancer Res
Identification of metabolic driver genes and targeted drug screening for lung adenocarcinoma metastasis at the single-cell resolution. [Abstract]2025 Aug 31;14(8):4774-4790. PMID: 40950658
Lösungsmittel & Löslichkeit
DMSO : ≥ 50 mg/mL (122.38 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
0.1 M NaOH : 33.33 mg/mL (81.58 mM; ultrasonic and adjust pH to 9 with NaOH)
H2O : < 0.1 mg/mL (insoluble)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.12 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.12 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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-
-
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Li Y, et al. Mechanism of hepatic targeting via oral administration of DSPE-PEG-Cholic acid-modified nanoliposomes. Int J Nanomedicine. 2017 Feb 28;12:1673-1684. [Content Brief]
[2]. Pan X, et al. Cholic acid Feeding Leads to Increased CYP2D6 Expression in CYP2D6-Humanized Mice. Drug Metab Dispos. 2017 Apr;45(4):346-352. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| 0.1 M NaOH / DMSO | 1 mM | 2.4476 mL | 12.2378 mL | 24.4756 mL | 61.1890 mL |
| 5 mM | 0.4895 mL | 2.4476 mL | 4.8951 mL | 12.2378 mL | |
| 10 mM | 0.2448 mL | 1.2238 mL | 2.4476 mL | 6.1189 mL | |
| 15 mM | 0.1632 mL | 0.8159 mL | 1.6317 mL | 4.0793 mL | |
| 20 mM | 0.1224 mL | 0.6119 mL | 1.2238 mL | 3.0595 mL | |
| 25 mM | 0.0979 mL | 0.4895 mL | 0.9790 mL | 2.4476 mL | |
| 30 mM | 0.0816 mL | 0.4079 mL | 0.8159 mL | 2.0396 mL | |
| 40 mM | 0.0612 mL | 0.3059 mL | 0.6119 mL | 1.5297 mL | |
| 50 mM | 0.0490 mL | 0.2448 mL | 0.4895 mL | 1.2238 mL | |
| 60 mM | 0.0408 mL | 0.2040 mL | 0.4079 mL | 1.0198 mL | |
| 80 mM | 0.0306 mL | 0.1530 mL | 0.3059 mL | 0.7649 mL | |
| DMSO | 100 mM | 0.0245 mL | 0.1224 mL | 0.2448 mL | 0.6119 mL |