DD1
Based on 1 Customer Validation
DD1, a proteasome inhibitor, targets Bax activation and P70S6K degradation during acute myeloid leukemia (AML) apoptosis. DD1 induces apoptosis in the caspase-dependent manner. DD1 induces mitochondrial membrane depolarization and Bad dephosphorylation.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.40%
- CAS. Nr.: 187585-11-1
- Formel: C16H14N2O3
- Molecular Weight:282.29
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Speicherung:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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VR23Trypsin-like/Chymotrypsin-like/Caspase-like proteasomes Inhibitor -
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Biologische Aktivität
Beschreibung
IC50 & Target
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p70S6K |
In Vitro
DD1 (20 μM; 96 h) shows antiproliferation activity in a dose- and time-dependent manner in U937 cells[1].
DD1 induces cell cycle arrest at G2/M phase and apoptosis[1].
DD1 (10, 20 μM; 48 h) induces mitochondrial membrane depolarization, Bax upregulation and Bad dephosphorylation[1].
DD1 (2-20 μM; 40 h) shows inhibitor for chymotrypsin-like activity in U937 lysates[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 187585-11-1
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Appearance Solid
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Molecular Weight 282.29
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Formel C16H14N2O3
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Color Light yellow to yellow
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SMILES
O=C1C(N)=C(C2=CC=C(OC)C(N)=C2)OC3=CC=CC=C13
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (354.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.86 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (274 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.5425 mL | 17.7123 mL | 35.4246 mL | 88.5614 mL |
| 5 mM | 0.7085 mL | 3.5425 mL | 7.0849 mL | 17.7123 mL | |
| 10 mM | 0.3542 mL | 1.7712 mL | 3.5425 mL | 8.8561 mL | |
| 15 mM | 0.2362 mL | 1.1808 mL | 2.3616 mL | 5.9041 mL | |
| 20 mM | 0.1771 mL | 0.8856 mL | 1.7712 mL | 4.4281 mL | |
| 25 mM | 0.1417 mL | 0.7085 mL | 1.4170 mL | 3.5425 mL | |
| 30 mM | 0.1181 mL | 0.5904 mL | 1.1808 mL | 2.9520 mL | |
| 40 mM | 0.0886 mL | 0.4428 mL | 0.8856 mL | 2.2140 mL | |
| 50 mM | 0.0708 mL | 0.3542 mL | 0.7085 mL | 1.7712 mL | |
| 60 mM | 0.0590 mL | 0.2952 mL | 0.5904 mL | 1.4760 mL | |
| 80 mM | 0.0443 mL | 0.2214 mL | 0.4428 mL | 1.1070 mL | |
| 100 mM | 0.0354 mL | 0.1771 mL | 0.3542 mL | 0.8856 mL |
DD1 Related Classifications
HY-120035 Related Classifications
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