5-DTAF
Based on 1 Customer Validation
5-DTAF is a fluorescein dye that directly react with polysaccharides and other alcohols in aqueous solution at pH ＞9. The maximum of the 5-DTAF emission profile is at a wavelength of 518 nm after excitation at 488 nm. 5-DTAF can be used for biomolecular labeling, protein detection, and cell imaging.
For research use only. We do not sell to patients.
- Purity: 95.74%
- CAS No.: 51306-35-5
- Formula: C23H12Cl2N4O5
- Molecular Weight:495.27
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
5-DTAF (10-5 mol/L; 1 h) acts as a fluorescent competitor for atrazine binding to Fe3O4-chitosan-based MMIP, with supernatant fluorescence intensity increases linearly correlated to the logarithm of atrazine concentration over 2.32-185.4 μM, enabling Atrazine (HY-N7091) detection[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Take cell labeling as an example:
(1) Wash the cells with PBS 3 times to remove serum and culture medium.
(2) Add serum-free medium containing 5-DTAF (1-5 μM), and incubate at 37°C in the dark for 30 minutes.
(3) Discard the staining solution, wash 3 times with preheated medium, and detect with a fluorescence microscope or flow cytometer.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
525
488
Chemical Information
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CAS No. 51306-35-5
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Appearance Solid
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Molecular Weight 495.27
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Formula C23H12Cl2N4O5
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Color Light yellow to yellow
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SMILES
O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=C(NC6=NC(Cl)=NC(Cl)=N6)C=C5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 50 mg/mL (100.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0191 mL | 10.0955 mL | 20.1910 mL | 50.4775 mL |
| 5 mM | 0.4038 mL | 2.0191 mL | 4.0382 mL | 10.0955 mL | |
| 10 mM | 0.2019 mL | 1.0096 mL | 2.0191 mL | 5.0478 mL | |
| 15 mM | 0.1346 mL | 0.6730 mL | 1.3461 mL | 3.3652 mL | |
| 20 mM | 0.1010 mL | 0.5048 mL | 1.0096 mL | 2.5239 mL | |
| 25 mM | 0.0808 mL | 0.4038 mL | 0.8076 mL | 2.0191 mL | |
| 30 mM | 0.0673 mL | 0.3365 mL | 0.6730 mL | 1.6826 mL | |
| 40 mM | 0.0505 mL | 0.2524 mL | 0.5048 mL | 1.2619 mL | |
| 50 mM | 0.0404 mL | 0.2019 mL | 0.4038 mL | 1.0096 mL | |
| 60 mM | 0.0337 mL | 0.1683 mL | 0.3365 mL | 0.8413 mL | |
| 80 mM | 0.0252 mL | 0.1262 mL | 0.2524 mL | 0.6310 mL | |
| 100 mM | 0.0202 mL | 0.1010 mL | 0.2019 mL | 0.5048 mL |
5-DTAF Related Classifications
HY-D1082 Related Classifications
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