Direct Red 80
Based on 2 publication(s) in Google Scholar
Direct Red 80 (Sirius Red) is a polyazo dye used principally in staining methods for collagen and amyloid. Direct Red 80 does not release benzidine upon degradation and is safer than many traditional direct dyes.
For research use only. We do not sell to patients.
- Purity: 95.0%
- CAS No.: 2610-10-8
- Formula: C45H26N10Na6O21S6
- Molecular Weight:1373.07
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Direct Red 80
More
Biological Activity
Direct Red 80 (Sirius Red) has two important uses in histopathology: staining collagen from solution in saturated aqueous picric acid and staining amyloid from an alkaline solution. Stained structures are red[1].
Confocal laser microscopy (CLM) is also used to evaluate collagen production and distribution after Direct Red 80 staining. Direct Red 80 is not specific for the different types of collagen. Samples are washed in dH2O, pretreats for 40 min with a solution of 0.2% phosphomolybdic acid (PMA) and incubates for 30 min in a solution consisting of 0.1% Direct red 80 diluted in saturated aqueous picric acid. Finally each sample is washed in 0.01 M HCl for 2 min. PMA is used to make colourless cytoplasm and HCl is used to remove non-specific staining[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2610-10-8
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Appearance Solid
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Molecular Weight 1373.07
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Formula C45H26N10Na6O21S6
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Color Brown to reddish brown
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SMILES
O=C(NC1=CC2=CC(S(=O)(O[Na])=O)=C(/N=N/C3=CC=C(/N=N/C4=CC=C(S(=O)(O[Na])=O)C=C4)C=C3S(=O)(O[Na])=O)C(O)=C2C=C1)NC5=CC6=CC(S(=O)(O[Na])=O)=C(/N=N/C7=CC=C(/N=N/C8=CC=C(S(=O)(O[Na])=O)C=C8)C=C7S(=O)(O[Na])=O)C(O)=C6C=C5
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Synonyms
Sirius Red
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (2)
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Journal Impact Factor
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Most Recent
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J Extracell Vesicles
Umbilical Cord-Mesenchymal Stromal Cell-Derived Extracellular Vesicles Target the Liver to Improve Neurovascular Health in Type 2 Diabetes With Non-Alcoholic Fatty Liver Disease. [Abstract]2025 Jul;14(7):e70125. PMID: 40620065 -
Solvent & Solubility
DMSO : 5 mg/mL (3.64 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (271 KB)
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SDS (537 KB)
- English - EN (537 KB)
- Français - FR (537 KB)
- Deutsch - DE (537 KB)
- Norwegian - NO (537 KB)
- Español - ES (537 KB)
- Swedish - SV (537 KB)
- Italian - IT (537 KB)
- Portuguese - PT (537 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.7283 mL | 3.6415 mL | 7.2830 mL | 18.2074 mL |
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