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Cat. No.: HY-116053

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PU-11

PU-11 is a Hsp90α/Hsp90β inhibitor with IC₅₀ values of 18.6 μM and 89.8 μM and K_d values of 2 and 4.2 μM. PU-11 binds to the ATP-binding pocket of Hsp90α and Hsp90β and displays selective binding preference for Hsp90α over Hsp90β, mediated by the nonconserved Hsp90α Ser52 residue. PU-11 can be used for the research of neurodegenerative disorders.

For research use only. We do not sell to patients.

CAS No.: 1454619-18-1
Formula: C₁₉H₂₃N₅O₃
Molecular Weight:369.43

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

PU-11 Chemical Structure

CAS No.: 1454619-18-1

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Protocols (1)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

IC₅₀ & Target

^[1]^[2]

HSP90α

18.6 μM (IC₅₀)

HSP90β

89.8 μM (IC₅₀)

HSP90α

2 μM (Kd)

HSP90β

4.2 μM (Kd)

All HSP Isoforms :

In Vitro

PU-11 (Compound PU-11-trans) (30.1-50.7 μM) binds to purified human Hsp90α NTD with a K_d of 2.0 ± 0.14 μM and an IC₅₀ of 18.6 μM, exhibiting selective affinity for this paralog^[1].
PU-11 (93.3-97.8 μM) binds to purified human Hsp90β NTD with a K_d of 4.2 ± 0.74 μM and an IC₅₀ of 89.8 μM, showing lower affinity than for Hsp90α^[1].
PU-11 (33.0-41.8 μM) binds to purified human Hsp90α^S52A mutant NTD with a K_d of 4.2 ± 0.03 μM, matching its affinity for wild-type Hsp90β^[1].
PU-11 shows IC₅₀ values of 111.4 ± 9.7 and 172.9 ± 7.3 μM for Grp94 and Trap-1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Chemical Information

CAS No. 1454619-18-1
Molecular Weight 369.43
Formula C₁₉H₂₃N₅O₃
SMILES

C(/C=C/C)N1C=2C(N=C1CC3=CC(OC)=C(OC)C(OC)=C3)=C(N)N=CN2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Huck JD, et al. Structures of Hsp90α and Hsp90β bound to a purine-scaffold inhibitor reveal an exploitable residue for drug selectivity. Proteins. 2019;87(10):869-877. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

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100 mg

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