Cat. No.: HY-N0222

Avicularin

9 Cited Publications

Customer Review

Based on 9 publication(s) in Google Scholar

Avicularin is an orally active flavonoid. Avicularin inhibits NF-κB (p65), COX-2 and PPAR-γ activities. Avicularin has anti-inflammatory, anti-infectious anti-allergic, anti-oxidant, hepatoprotective, and anti-tumor activities.

For research use only. We do not sell to patients.

Purity: 99.80%
CAS No.: 572-30-5
Formula: C₂₀H₁₈O₁₁
Molecular Weight:434.35

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Avicularin Chemical Structure

CAS No.: 572-30-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (9)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[2]^[3]

GLUT4

PPARγ

COX-2

All COX Isoforms :

All NF-κB Isoforms :

All PPAR Isoforms :

All ERK Isoforms :

All GLUT Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>10 μg/mL Compound: Compound 5	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 25862199]
HeLa	IC₅₀	>10 μg/mL Compound: Compound 5	Cytotoxicity against human HeLa cells by MTT assay Cytotoxicity against human HeLa cells by MTT assay	[PMID: 25862199]
SGC-7901	IC₅₀	>10 μg/mL Compound: Compound 5	Cytotoxicity against human SGC7901 cells by MTT assay Cytotoxicity against human SGC7901 cells by MTT assay	[PMID: 25862199]

In Vitro

Avicularin (10-300 μM, 1 h) suppresses NO and PGE₂ production in LPS-stimulated RAW 264.7 cells^[1].
? Avicularin (10-300 μM, 1 h) exhibits anti-inflammatory activity through the suppression of ERK signaling pathway in LPS-stimulated RAW 264.7 cells^[1].
? Avicularin (25-100 μg/mL, 48 h) decreases cell proliferation, cell migration and invasion in Huh7 cells^[2].
? Avicularin (25-100 μg/mL, 48 h) induces apoptosis via the downregulation of NF?κB (p65) and COX?2 and the upregulation of PPAR?γ^[2].
? Avicularin (50 μM, 6 days) decreases the intracellular lipids, along with decreased PPARγ, C/EBPα, and aP2 mRNA levels in 3T3-L1 cells^[3].
? Avicularin (50 μM, 6 days) suppresses GLUT4-Mediated glucose uptake in 3T3-L1 cells^[3].
? Avicularin (2.5-10 μM, 2 h) inhibits ECM degradation and inflammation via TRAF6/MAPK activation in rat and human chondrocytes^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:

Huh7 cells
Concentration:

25, 50, 100 μg/mL
Incubation Time:

12, 24, 36 and 48 h
Result:

Inhibited cell proliferation in a dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:

RAW 264.7 cells
Concentration:

10, 30, 100, 300 μM
Incubation Time:

1 h
Result:

Inhibited LPS-induced protein expression of iNOS and COX-2, release of pro-inflammatory cytokine IL-1β, degradation of cytosolic IκB, and phosphorylation of ERK.

RT-PCR^[3]

Cell Line:

3T3-L1 cells
Concentration:

50 μM
Incubation Time:

6 days
Result:

Decreased PPARγ, C/EBPα, and aP2 mRNA levels approximately 28.7, 69.5, and 18.3%, respectively.

In Vivo

Avicularin (injected in articular cavity of the knee, 0.5-2 mg/kg, twice a week for 4 weeks) attenuates the development of OA (osteoarthritis) in ACLT-induced rats^[4].
? Avicularin (oral administration, 50 and 100 mg/kg, for 21 days) attenuates memory Impairment in rats with amyloid Beta-induced Alzheimer's disease^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

ACLT (anterior cruciate ligament transection)-induced rats^[4]
Dosage:

0.5, 1, 2 mg/kg
Administration:

Injected into the articular cavity of the right knee, twice a week for 4 weeks.
Result:

Alleviated the tibial subchondral osteolysis, reduces bone loss, and increases the bone mass of tibial subchondral bone.
Attenuated ECM degradation, the loss of Aggrecan and Collagen II in ACLT-induced rats.
Decreased the MMP3 and MMP13 protein level.

Animal Model:

Rats with amyloid Beta-induced Alzheimer's disease^[5]
Dosage:

25, 50, and 100 mg/kg
Administration:

Oral administration, for 21 days
Result:

Enhanced cognition activity, and reversed the effects of amyloid beta-induced inflammatory response and excessive oxidative stress.

Chemical Information

CAS No. 572-30-5
Appearance Solid
Molecular Weight 434.35
Formula C₂₀H₁₈O₁₁
Color Light yellow to yellow
SMILES

O=C1C(O[C@H]2[C@@H]([C@H]([C@H](CO)O2)O)O)=C(C3=CC=C(O)C(O)=C3)OC4=CC(O)=CC(O)=C14
Structure Classification
- Flavonoids
- Flavonols
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (9)

All Cell Proliferation/Viability AssayIFWB

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Journal Impact Factor

Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530
Biomed Pharmacother

Avicularin alleviates osteoporosis-induced implant loosening by attenuating macrophage M1 polarization via its inhibitory effect on the activation of NF-κB. [Abstract]

2023 Feb:158:114113. PMID: 36516692
Ind Crops Prod

2026 May 8;246:123392.
Ind Crops Prod

2025 Dec 11;239:122458.
J Agric Food Chem

Avicularin Alleviates Osteoporosis in Ovariectomized Mice by Inhibiting Osteoclastogenesis through NF-κB Pathway Inhibition. [Abstract]

2023 Jan 11;71(1):411-420. PMID: 36540936

Avicularin purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2023 Jan 11;71(1):411-420. [Abstract]

Avicularin (AL; 10, 30, 100, 300 μM; 5 days) disrupts RANKL-induced osteoclastogenesis in vitro. Representative image of in vitro TRAP staining.

Avicularin purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2023 Jan 11;71(1):411-420. [Abstract]

Avicularin (AL; 10, 100, 300 μM) decreases F-actin ring size and number and nuclei count in a dose-dependent manner.

Avicularin purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2023 Jan 11;71(1):411-420. [Abstract]

Avicularin (AL; 100 μM; 0, 10, 20, 30, and 60 min) disrupts RANKL-induced osteoclast differentiation by inhibiting the NF-κB signaling pathway (Fig A). Avicularin (AL; 100 μM; 0, 10, 20, 30, and 60 min) also disrupts IκBα phosphorylation and degradation (Fig B–D).
Pestic Biochem Physiol

Mechanistic insights into chlorogenic acid and caffeic acid as novel juvenile hormone antagonists. [Abstract]

2025 Dec:215:106655. PMID: 41162045
Aging (Albany NY)

Fisetin inhibits the proliferation, migration and invasion of pancreatic cancer by targeting PI3K/AKT/mTOR signaling. [Abstract]

2021 Nov 25;13(22):24753-24767. PMID: 34821587
J Integr Neurosci

Avicularin Treatment Ameliorates Ischemic Stroke Damage by Regulating Microglia Polarization and its Exosomes via the NLRP3 Pathway. [Abstract]

2024 Oct 30;23(11):196. PMID: 39613475

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (230.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3023 mL

11.5115 mL

23.0229 mL

5 mM

0.4605 mL

2.3023 mL

4.6046 mL

10 mM

0.2302 mL

1.1511 mL

2.3023 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (290 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Vo VA, et al. Avicularin Inhibits Lipopolysaccharide-Induced Inflammatory Response by Suppressing ERK Phosphorylation in RAW 264.7 Macrophages. Biomol Ther (Seoul). 2012 Nov;20(6):532-7. [Content Brief]

[2]. Wang Z, et al. Avicularin ameliorates human hepatocellular carcinoma via the regulation of NF‑κB/COX‑2/PPAR‑γ activities. Mol Med Rep. 2019 Jun;19(6):5417-5423. [Content Brief]

[3]. Ko Fujimori, et al. Avicularin, a plant flavonoid, suppresses lipid accumulation through repression of C/EBPα-activated GLUT4-mediated glucose uptake in 3T3-L1 cells. J Agric Food Chem. 2013 May 29;61(21):5139-47. [Content Brief]

[4]. Zi-Ling Zou, et al. Avicularin suppresses cartilage extracellular matrix degradation and inflammation via TRAF6/MAPK activation. Phytomedicine. 2021 Oct;91:153657. [Content Brief]

[5]. Nikita Patil Samant, et al. Avicularin Attenuates Memory Impairment in Rats with Amyloid Beta-Induced Alzheimer's Disease. Neurotox Res. 2022 Feb;40(1):140-153. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3023 mL	11.5115 mL	23.0229 mL	57.5573 mL
	5 mM	0.4605 mL	2.3023 mL	4.6046 mL	11.5115 mL
	10 mM	0.2302 mL	1.1511 mL	2.3023 mL	5.7557 mL
	15 mM	0.1535 mL	0.7674 mL	1.5349 mL	3.8372 mL
	20 mM	0.1151 mL	0.5756 mL	1.1511 mL	2.8779 mL
	25 mM	0.0921 mL	0.4605 mL	0.9209 mL	2.3023 mL
	30 mM	0.0767 mL	0.3837 mL	0.7674 mL	1.9186 mL
	40 mM	0.0576 mL	0.2878 mL	0.5756 mL	1.4389 mL
	50 mM	0.0460 mL	0.2302 mL	0.4605 mL	1.1511 mL
	60 mM	0.0384 mL	0.1919 mL	0.3837 mL	0.9593 mL
	80 mM	0.0288 mL	0.1439 mL	0.2878 mL	0.7195 mL
	100 mM	0.0230 mL	0.1151 mL	0.2302 mL	0.5756 mL

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Avicularin

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