BD-105 

BD-105 is a glucagon-binding fluorescent probe with a K_a value of 13.3 μM. BD-105 exhibits changes in fluorescence intensity upon interaction with glucagon, and colocalizes with glucagon in cells and tissues. BD-105 is a selective cell stain that labels glucagon-secreting cells without staining insulin-secreting cells or non-endocrine control cells. BD-105 serves as an imaging reagent for glucagon in live cells and tissues^[1].

BD-105 (500 nM; 24 h) selectively stains glucagon-secreting AlphaTC1 cells, with no detectable staining in BetaTC6 or HeLa cells^[1].
BD-105 (10 μM) in buffer has an absorption maximum of 550 nm and emission maximum of 568 nm with a quantum yield of ~0.012; upon addition of 200 μM glucagon to 1 μM BD-105, quantum yield increases to 0.151 with absorption and emission maxima shifted to 553 nm and 570 nm, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

324.13

C₁₈H₁₅BF₂N₂O

1180112-32-6

[F-][B+3]1([N]2=CC=CC2=CC3=C(C)C=C([N-]31)/C=C/C4=CC(O)=CC=C4)[F-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Lee JS, et al. Synthesis of a BODIPY library and its application to the development of live cell glucagon imaging probe. J Am Chem Soc. 2009;131(29):10077-10082.  [Content Brief]