BI-836858
Based on 1 Customer Validation
BI-836858 is a fully human anti-CD33 monoclonal antibody. BI-836858 blocks CD33-mediated inhibitory signaling pathways in MDS bone marrow cells and reduces the expression of IL-10 and TGFβ. BI-836858 decreases the secretion of immunosuppressive cytokines and reactive oxygen species (ROS)-induced genomic instability, restoring bone marrow hematopoietic function. BI-836858 can be used for research on myelodysplastic syndromes (MDS) and AML.
For research use only. We do not sell to patients.
- Purity : 95.81%
- Molecular Weight:143.86 kDa
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Storage:Shipping with dry ice
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Human IgG1 kappa
Recommend Isotype Controls
Species Reactivity
Human
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CD33 |
IL-10 |
In Vitro
BI-836858 (2 μg/mL; 4 h) mediates NK cell-dependent ADCC activity against CD33-positive HL60 cells[2].
BI-836858 (2 μg/mL; 48 h) blocks the CD33-mediated inhibitory signaling pathway in MDS bone marrow cells and reduces the expression of IL-10 and TGFβ[2].
BI-836858 (2 μg/mL) specifically blocks CD33 (S100A9)-mediated ROS production in U937 cells, healthy PBMCs, and primary MDS BM HSPCs[2].
BI-836858 (2 μg/mL; 14 days) restores the hematopoietic colony-forming capacity in ex vivo cultures of primary MDS bone marrow specimens[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Application
ELISA, FACS, Functional assay
Verified Bioactivity
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Immobilized huCD33-his can bind BI-836858. The EC50 for this effect is 9.98 ng/mL.
Chemical Information
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Appearance Liquid
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Molecular Weight 143.86 kDa
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Color Colorless to light yellow
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SMILES
N/A
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Gopalakrishnan B, et al. Polo-like kinase inhibitor volasertib marginally enhances the efficacy of the novel Fc-engineered anti-CD33 antibody BI 836858 in acute myeloid leukemia. Oncotarget. 2018;9(11):9706-9713. Published 2018 Jan 3. [Content Brief]
[2]. Eksioglu EA, et al. Novel therapeutic approach to improve hematopoiesis in low risk MDS by targeting MDSCs with the Fc-engineered CD33 antibody BI 836858. Leukemia. 2017;31(10):2172-2180. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
BI-836858 Related Classifications
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