Biotin-PEG2-C4-alkyne
Biotin-PEG2-C4-alkyne is a PEG-based PROTAC linker can be used in the synthesis of PROTACs. Biotin-PEG2-C4-alkyne is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- CAS No.: 1011268-28-2
- Formula: C22H36N4O5S
- Molecular Weight:468.61
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
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Help & FAQs
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HY-130143
Biotin-PEG3-azidePROTAC Linker -
HY-140922
Biotin-PEG4-alkynePROTAC Linker -
HY-140928
Biotin-PEG4-amino-t-Bu-DADPS-C3-alyknePROTAC Linkers -
HY-140924
Dde Biotin-PEG4-alkynePROTAC Linker -
HY-133870
Itaconate-alkyneBioorthogonal Probe -
HY-140923
Biotin-PEG4-amide-AlkynePROTAC Linker -
HY-100215
Biotin HydrazideCarbonyl Probe -
HY-140925
Diazo Biotin-PEG3-alkynePROTAC Linker -
HY-140130
PC Biotin-PEG3-alkyneADC Linker -
HY-100215R
Biotin Hydrazide (Standard)Carbonyl Probe
All PROTAC Linkers Isoforms
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Biological Activity
Description
IC50 & Target
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PEGs |
In Vitro
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein.PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1011268-28-2
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Molecular Weight 468.61
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Formula C22H36N4O5S
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SMILES
O=C(NCCOCCOCCNC(CCCC#C)=O)CCCC[C@@H]1SC[C@]([C@]1([H])N2)([H])NC2=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Biotin-PEG2-C4-alkyne Related Classifications
HY-113828 Related Classifications
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