Biotin-PEG8-azide
Based on 1 Customer Validation
Biotin-PEG8-azide is a bioconjugation reagent. Biotin-PEG8-azide consists of biotin, PEG8 linker and azide group and serves as a modifying linker in click reactions. Biotin-PEG8-azide can be used for research related to click chemistry.
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- Purity : 98.77%
- CAS No.: 2363756-34-5
- 화학식: C28H52N6O10S
- 분자량:664.81
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보관:Pure form -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
In Vitro
Biotin-PEG8-azide can generate biotinylated compound 371 via click chemistry reaction; this compound inhibits the ClpXP protease of Staphylococcus aureus with an IC50 of 27 µM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 2363756-34-5
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Appearance Oil
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분자량 664.81
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화학식 C28H52N6O10S
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Color Off-white to light yellow
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SMILES
O=C(CCCC[C@@H]1SC[C@]([H])(N2)[C@@]1(NC2=O)[H])NCCOCCOCCOCCOCCOCCOCCOCCOCCN=[N+]=[N-]
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Pure form -20°C 3 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
In Vitro:
DMSO : 125 mg/mL (188.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
순도&문서
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Data Sheet (265 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5042 mL | 7.5209 mL | 15.0419 mL | 37.6047 mL |
| 5 mM | 0.3008 mL | 1.5042 mL | 3.0084 mL | 7.5209 mL | |
| 10 mM | 0.1504 mL | 0.7521 mL | 1.5042 mL | 3.7605 mL | |
| 15 mM | 0.1003 mL | 0.5014 mL | 1.0028 mL | 2.5070 mL | |
| 20 mM | 0.0752 mL | 0.3760 mL | 0.7521 mL | 1.8802 mL | |
| 25 mM | 0.0602 mL | 0.3008 mL | 0.6017 mL | 1.5042 mL | |
| 30 mM | 0.0501 mL | 0.2507 mL | 0.5014 mL | 1.2535 mL | |
| 40 mM | 0.0376 mL | 0.1880 mL | 0.3760 mL | 0.9401 mL | |
| 50 mM | 0.0301 mL | 0.1504 mL | 0.3008 mL | 0.7521 mL | |
| 60 mM | 0.0251 mL | 0.1253 mL | 0.2507 mL | 0.6267 mL | |
| 80 mM | 0.0188 mL | 0.0940 mL | 0.1880 mL | 0.4701 mL | |
| 100 mM | 0.0150 mL | 0.0752 mL | 0.1504 mL | 0.3760 mL |
Biotin-PEG8-azide Related Classifications
HY-W190824 Related Classifications
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