Bovin beta-1,4-galactosyltransferase 1 (Y289L)
Based on 1 Customer Validation
Bovin beta-1,4-galactosyltransferase 1 (Y289L) (Bovin B4GALT1 (Y289L)) is a mutated form of bovine-derived galactosyltransferase with a mutation at the Y289L genetic site. Bovin beta-1,4-galactosyltransferase 1 can label O-GlcNAcylated proteins with an N-azidoacetylgalactosamine (GalNAz) group. This labeling method allows for the specific, unbiased, and global labeling of O-GlcNAcylated proteins. After labeling, the appended azide group can react with a wide variety of alkyne-modified chemical probes, facilitating multiple downstream analyses.
For research use only. We do not sell to patients.
- Molecular Weight:50.8 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
MCE Validation Data
In Vitro
Product Information
Bovin B4GALT1(Y289L) is a mutant galactosyltransferase capable of efficiently transferring GalNAc or GalNAz to terminal GlcNAc residues. The latter finds application in antibody-drug conjugates (ADCs) for targeted cancer therapy and other purposes.This product is recombinantly expressed using the HEK293 cell expression system, with a C-terminal His tag. It has a molecular weight of 50.8 kDa and is stored in a buffer consisting of 20 mM Tris-HCl (pH 7.4), 200 mM NaCl, and 20% glycerin.
Instructions
1. Reaction System:
50 mM Tris-HCl (pH 7.5), 6 mM MnCl2
2. Reaction Reagents:
UDP-GalNAz to Antibody Ratio: 300:1 (by equivalent)
Enzyme Addition Amount: Enzyme/antibody = 5%
3. Prepare the reaction solution according to the above conditions, react at 37°C for 48 h, then place at -15~-25°C to stop the reaction and perform sample testing.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
EC Number
2.4.1-
Specific Activity
2500 U/mg
Unit Definition
One unit is defined as the amount of enzyme that will transfer 1 nmol GalNAz from UDP-GalNAz to
Technical Parameters
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Source
HEK293 cells
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Tag
C-terminal His Tagged
Chemical Information
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Appearance Liquid
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Molecular Weight 50.8 kDa
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Color Colorless to light yellow
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SMILES
[Bovin beta-1,4-galactosyltransferase 1 (Y289L)]
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Synonyms
Bovin B4GALT1 (Y289L)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (267 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Bovin beta-1,4-galactosyltransferase 1 (Y289L) Related Classifications
HY-E70289 Related Classifications
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