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  5. Bz-Nle-Lys-Arg-Arg-AMC

Bz-Nle-Lys-Arg-Arg-AMC can be used for protease activity assay. In 0.1mL Tris-HCl buffer (50 mM, pH 7.8), Bz-Nle-Lys-Arg-Arg-AMC was hydrolyzed, and the release rate of AMC was observed.

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Bz-Nle-Lys-Arg-Arg-AMC

Bz-Nle-Lys-Arg-Arg-AMC Chemical Structure

CAS No. : 863975-32-0

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Based on 1 publication(s) in Google Scholar

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Description

Bz-Nle-Lys-Arg-Arg-AMC can be used for protease activity assay. In 0.1mL Tris-HCl buffer (50 mM, pH 7.8), Bz-Nle-Lys-Arg-Arg-AMC was hydrolyzed, and the release rate of AMC was observed[1].

Molecular Weight

832.99

Formula

C41H60N12O7

CAS No.
Appearance

Solid

Color

White to off-white

Sequence

{Bz-Nle}-Lys-Arg-Arg-AMC

Sequence Shortening

{Bz-Nle}-Lys-Arg-Arg-AMC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (30.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2005 mL 6.0025 mL 12.0049 mL
5 mM 0.2401 mL 1.2005 mL 2.4010 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2005 mL 6.0025 mL 12.0049 mL 30.0124 mL
5 mM 0.2401 mL 1.2005 mL 2.4010 mL 6.0025 mL
10 mM 0.1200 mL 0.6002 mL 1.2005 mL 3.0012 mL
15 mM 0.0800 mL 0.4002 mL 0.8003 mL 2.0008 mL
20 mM 0.0600 mL 0.3001 mL 0.6002 mL 1.5006 mL
25 mM 0.0480 mL 0.2401 mL 0.4802 mL 1.2005 mL
30 mM 0.0400 mL 0.2001 mL 0.4002 mL 1.0004 mL
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Bz-Nle-Lys-Arg-Arg-AMC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Bz-Nle-Lys-Arg-Arg-AMC
Cat. No.:
HY-P2435
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