Digoxigenin NHS ester
Based on 1 Customer Validation
Digoxigenin NHS ester is an activated ester which readily reacts with amino groups under mild conditions, attaching the Digoxigenin (HY-B1025) moiety to proteins or amino-. Digoxigenin NHS ester can be used to label proteins and oligonucleotides.
For research use only. We do not sell to patients.
- Purity: 99.00%
- CAS No.: 129273-26-3
- Formula: C35H50N2O10
- Molecular Weight:658.78
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Digoxigenin NHS ester (1 mg/mL, overnight) after reacting with R-phycocyanin solution can serve as the donor in the fluorescence energy transfer (FRET) system, enabling highly sensitive detection of Digoxin (HY-B1049)[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 129273-26-3
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Appearance Solid
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Molecular Weight 658.78
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Formula C35H50N2O10
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Color White to off-white
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SMILES
O[C@@]12[C@@]([C@@H](C(CO3)=CC3=O)CC1)([C@@H](C[C@@]4([H])[C@@]2([H])CC[C@@]5([H])[C@@]4(CC[C@@H](C5)OCC(NCCCCCC(ON6C(CCC6=O)=O)=O)=O)C)O)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (151.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
- Norwegian - NO (644 KB)
- Español - ES (644 KB)
- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Korean - KR (644 KB)
- Portuguese - PT (644 KB)
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Handling Instructions (2659 KB)
References
[2]. Dalia BURZYN, et al. Extracellular vesicle-aso constructs targeting stat6. Patent, WO2021030776A1.
[3]. Graefe KA, et al. High-performance liquid chromatography with on-line post-column immunoreaction detection of digoxin and its metabolites based on fluorescence energy transfer in the far-red spectral region. J Chromatogr B Biomed Sci Appl. 2000 Aug 18;745(2):305-14. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5180 mL | 7.5898 mL | 15.1796 mL | 37.9489 mL |
| 5 mM | 0.3036 mL | 1.5180 mL | 3.0359 mL | 7.5898 mL | |
| 10 mM | 0.1518 mL | 0.7590 mL | 1.5180 mL | 3.7949 mL | |
| 15 mM | 0.1012 mL | 0.5060 mL | 1.0120 mL | 2.5299 mL | |
| 20 mM | 0.0759 mL | 0.3795 mL | 0.7590 mL | 1.8974 mL | |
| 25 mM | 0.0607 mL | 0.3036 mL | 0.6072 mL | 1.5180 mL | |
| 30 mM | 0.0506 mL | 0.2530 mL | 0.5060 mL | 1.2650 mL | |
| 40 mM | 0.0379 mL | 0.1897 mL | 0.3795 mL | 0.9487 mL | |
| 50 mM | 0.0304 mL | 0.1518 mL | 0.3036 mL | 0.7590 mL | |
| 60 mM | 0.0253 mL | 0.1265 mL | 0.2530 mL | 0.6325 mL | |
| 80 mM | 0.0190 mL | 0.0949 mL | 0.1897 mL | 0.4744 mL | |
| 100 mM | 0.0152 mL | 0.0759 mL | 0.1518 mL | 0.3795 mL |
Digoxigenin NHS ester Related Classifications
HY-155500 Related Classifications
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