5-JOE azide
5-JOE azide is a fluorescent dye with the emission in yellow region of the spectrum. The azide group can react with alkyne, BCN, DBCO via Click Chemistry to yield a stable triazole linkage.
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- CAS No.: 1422178-11-7
- Formule: C26H20Cl2N4O8
- Masse moléculaire:587.37
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
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Help & FAQs
Activité biologique
Description
Chemical Information
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CAS No. 1422178-11-7
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Masse moléculaire 587.37
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Formule C26H20Cl2N4O8
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SMILES
[N-]=[N+]=NCCCNC(C1=CC=C2C(C(OC23C4=CC(OC)=C(C(Cl)=C4OC5=C(C(O)=C(C=C53)OC)Cl)O)=O)=C1)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Pureté et documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
5-JOE azide Related Classifications
HY-D2748 Related Classifications
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