Cat. No.: HY-N0519

Calycosin

(Synonyms: Cyclosin)

22 Cited Publications

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Based on 22 publication(s) in Google Scholar

Calycosin is a compound that can be isolated from Radix Astragali. Calycosin has strong antioxidant, anti-inflammatory and apoptosis-modulating effects. Calycosin can be used for the research of ovarian cancer and breast cancer.

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Pureté: 99.96%
CAS No.: 20575-57-9
Formule: C₁₆H₁₂O₅
Masse moléculaire:284.26

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Calycosin Chemical Structure

CAS No.: 20575-57-9

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Activité biologique
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (22)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>100 μM Compound: 14	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 18440233]
B16-BL6	IC₅₀	>100 μM Compound: 14	Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay	[PMID: 18440233]
BV-2	IC₅₀	39.56 μM Compound: 6	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay	[PMID: 28911817]
HeLa	IC₅₀	>100 μM Compound: 14	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 18440233]
HepG2	IC₅₀	>100 μM Compound: 7	Anticancer activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay Anticancer activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 24974349]
HT-1080	IC₅₀	>100 μM Compound: 14	Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 18440233]
Lewis lung carcinoma cell line	IC₅₀	>100 μM Compound: 14	Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay	[PMID: 18440233]
N9	IC₅₀	17.43 μM Compound: 5	Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 28073678]
PC-3	IC₅₀	192 μM Compound: 5	Cytotoxicity against human PC3 cells Cytotoxicity against human PC3 cells	[PMID: 16441066]
RAW264.7	IC₅₀	>50 μM Compound: 9	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay	[PMID: 25592708]
U-937	IC₅₀	43 μM Compound: 7, clycosin	Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay	[PMID: 17158054]
U-937	IC₅₀	48 μM Compound: 7, clycosin	Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay	[PMID: 17158054]
U-937	IC₅₀	49 μM Compound: 7, clycosin	Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay	[PMID: 17158054]
Vero	IC₅₀	159 μM Compound: 5	Cytotoxicity against african green monkey Vero cells Cytotoxicity against african green monkey Vero cells	[PMID: 16441066]

In Vitro

Calycosin (0-100 μM, 24-72 h) inhibits the proliferation of SKOV3 cells in a dose- and time-dependent manner^[1].
Calycosin (25-100 μM, 48 h) upregulates the Bax/Bcl-2 ratio and the expression of cleaved caspase-3 and cleaved caspase-9 in a dose-dependent manner in SKOV3 cells^[1].
Calycosin (0-16 μM, 0-240 min) stimulates rapid activation of ERK1/2 in a time- and dose-dependent manner in MCF-7 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:

MCF-7 cells
Concentration:

2-32 μM
Incubation Time:

48 h
Result:

Increased the MCF-7 cell number at the doses of 2-8 μM.
Inhibited the proliferation of MCF-7 cells at the doses of 32 μM.

In Vivo

Calycosin (1-4 mg/kg, i.p., daily, 20 days) inhibits the protein expression of ERα in OVX mice at the doses of 1 mg/kg^[2].
Calycosin (7.5-30 mg/kg, i.g., daily, 3 days) has a neuroprotective effect against cerebral ischemia/reperfusion injury in adult male Sprague-Dawley rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Adult Male Sprague-Dawley Rats^[3]
Dosage:

7.5-30 mg/kg
Administration:

i.g. daily, 3 days
Result:

Improved neurological deficit and infarct volum.
Reduced the content of malondialdehyde (MDA), protein carbonyl and reactive oxygen species (ROS).
Increased the activities of superoxide dismutase (SOD), catalase and glutathione peroxidase (GSH-Px) in a dose-dependent manner.
Inhibited the expression of 4-Hydroxy-2-nonenal (4-HNE).

Chemical Information

CAS No. 20575-57-9
Appearance Solid
Masse moléculaire 284.26
Formule C₁₆H₁₂O₅
Color White to off-white
SMILES

O=C1C(C2=CC=C(OC)C(O)=C2)=COC3=CC(O)=CC=C13
Synonyms

Cyclosin
Structure Classification
- Flavonoids
- Isoflavones
- Phenols
- Polyphenols
Initial Source
- Plants
- other families
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (22)

All Cell Imaging/StainingCell Proliferation/Viability AssayRT-PCRBio/Physico-chemical AssayHistological Imaging/StainingWB

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Journal Impact Factor

Acta Pharm Sin B

Chrysin serves as a novel inhibitor of DGK α/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). [Abstract]

2021 Jan;11(1):143-155. PMID: 33532186
Phytomedicine

Calycosin alleviates myocardial fibrosis after myocardial infarction by restoring fatty acid metabolism homeostasis through inhibiting FAP. [Abstract]

2025 Sep:145:157045. PMID: 40627929
Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530
Br J Pharmacol

The natural compound, formononetin, extracted from Astragalus membranaceus increases adipocyte thermogenesis by modulating PPARγ activity. [Abstract]

2018 May;175(9):1439-1450. PMID: 29315511

Calycosin purchased from MedChemExpress. Usage Cited in: Br J Pharmacol. 2018 May;175(9):1439-1450. [Abstract]

Calycosin (0-10 μM) dose dependently promoted luciferase activity in COS7 cells transfected with PPARγ and PPRE‐luciferase plasmids.

Calycosin purchased from MedChemExpress. Usage Cited in: Br J Pharmacol. 2018 May;175(9):1439-1450. [Abstract]

The effect of Calycosin (0-3 μM) on Ucp1 expression in primary differentiated inguinal adipocytes was analyzed using qPCR.\
Chin Med

Therapeutic potential of Sheng-Xian-Tang in doxorubicin-induced chronic heart failure by regulation of phenylalanine metabolism disruption. [Abstract]

2026 Jan 12;21(1):29. PMID: 41527105
J Ethnopharmacol

Discovery and validation of anti-arthritic ingredients and mechanisms of Qingfu Juanbi Tang, a Chinese herbal formulation, on rheumatoid arthritis. [Abstract]

2024 Jul 15:329:118140. PMID: 38565409
Biochem Pharmacol

Downregulation of KLF2 impairs pulmonary endothelial function and promotes persistent pulmonary hypertension of the newborn. [Abstract]

2025 Nov 27:244:117572. PMID: 41318102
Ind Crops Prod

2025 Dec 11;239:122458.
Pharm Biol

Calycosin plays a protective role in diabetic kidney disease through the regulation of ferroptosis. [Abstract]

2022 Dec;60(1):990-996. PMID: 35587919

Calycosin purchased from MedChemExpress. Usage Cited in: Pharm Biol. 2022 Dec;60(1):990-996. [Abstract]

The cell viability was measured in HK-2 cells treated with HG and different concentrations (0, 5, 10, 20, 40 and 80 μM; 48 h) of Calycosin.

Calycosin purchased from MedChemExpress. Usage Cited in: Pharm Biol. 2022 Dec;60(1):990-996. [Abstract]

The levels of LDH, MDA, GSH were measured in HK-2 cells treated with HG and different concentrations (0, 10, 20 and 40 μM) of calycosin.

Calycosin purchased from MedChemExpress. Usage Cited in: Pharm Biol. 2022 Dec;60(1):990-996. [Abstract]

HK-2 cells were treated with erastin (35 μM) and/or Calycosin (40 μM). Then, the lipid ROS levels were detected.

Calycosin purchased from MedChemExpress. Usage Cited in: Pharm Biol. 2022 Dec;60(1):990-996. [Abstract]

Calycosin plays a protective role in db/db mouse through the inhibition of ferroptosis. The db/db mice were randomly divided into a db/db group, a db/db plus 10 mg/kg/day Calycosin group, and a db/db plus 20 mg/kg/day Calycosin group. The db/m mice served as controls. Calycosin or saline was intraperitoneally injected into the mice for 4 weeks. HE and Masson’s Trichrome staining of kidney sections.

Calycosin purchased from MedChemExpress. Usage Cited in: Pharm Biol. 2022 Dec;60(1):990-996. [Abstract]

Calycosin plays a protective role in db/db mouse through the inhibition of ferroptosis. The db/db mice were randomly divided into a db/db group, a db/db plus 10 mg/kg/day Calycosin group, and a db/db plus 20 mg/kg/day Calycosin group. The db/m mice served as controls. Calycosin or saline was intraperitoneally injected into the mice for 4 weeks. Protein levels of GPX4 and NCOA4 were detected.
Int Immunopharmacol

Minimolide F alleviates inflammatory diseases by specifically targeting STING and blocking IRF3 recruitment. [Abstract]

2026 May 1:176:116468. PMID: 41819671
Drug Dev Res

Calycosin represses AIM2 inflammasome-mediated inflammation and pyroptosis to attenuate monosodium urate-induced gouty arthritis through NF-κB and p62-Keap1 pathways. [Abstract]

2022 Nov;83(7):1654-1672. PMID: 36069386
J Funct Foods

2024 Apr, 115, 106087.
J Neuroimmunol

Calycosin regulates astrocyte reactivity and astrogliosis after spinal cord injury by targeting STAT3 phosphorylation. [Abstract]

2025 Jan 30:400:578535. PMID: 39954615
BMC Nephrol

Calycosin ameliorates albuminuria in nephrotic syndrome by targeting Notch1/Snail pathway. [Abstract]

2025 Apr 18;26(1):198. PMID: 40251522
J Nat Med

Calycosin ameliorates inflammation and M1 macrophage polarization via Spon2 in LPS-triggered MH-S alveolar macrophages. [Abstract]

2025 Oct 6. PMID: 41053324
In Vitro Cell Dev Biol Anim

Calycosin prevents IL-1β-induced articular chondrocyte damage in osteoarthritis through regulating the PI3K/AKT/FoxO1 pathway. [Abstract]

2022 Jun;58(6):491-502. PMID: 35705795
Rev Bras Farmacogn

2023 Aug 14.
Xenobiotica

Calycosin improves insulin resistance by regulating the hsa-miR-324-3p/AKT pathway to inhibit FOXO3a nuclear transfer. [Abstract]

2025 Sep 18:1-9. PMID: 40924558
bioRxiv

2026 May 10.
SSRN

2025 Jul 10.
Evid Based Complement Alternat Med

Calycosin Alleviates Injury in Airway Epithelial Cells Caused by PM 2.5 Exposure via Activation of AMPK Signalling. [Abstract]

2021 May 4:2021:8885716. PMID: 34055025

Solvant et solubilité

In Vitro:

DMSO : ≥ 100 mg/mL (351.79 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.5179 mL

17.5895 mL

35.1791 mL

5 mM

0.7036 mL

3.5179 mL

7.0358 mL

10 mM

0.3518 mL

1.7590 mL

3.5179 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (8.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (8.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (8.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (277 KB)
SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
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- Portuguese - PT (479 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Zhou Y, et al. Calycosin induces apoptosis in human ovarian cancer SKOV3 cells by activating caspases and Bcl-2 family proteins. Tumour Biol. 2015 Feb 12. [Content Brief]

[2]. Chen J, et al. Calycosin promotes proliferation of estrogen receptor-positive cells via estrogen receptors and ERK1/2 activation in vitro and in vivo. Cancer Lett. 2011 Sep 28;308(2):144-51. [Content Brief]

[3]. Guo C, et al. Neuroprotective effect of calycosin on cerebral ischemia and reperfusion injury in rats. J Ethnopharmacol. 2012 Dec 18;144(3):768-74. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.5179 mL	17.5895 mL	35.1791 mL	87.9477 mL
	5 mM	0.7036 mL	3.5179 mL	7.0358 mL	17.5895 mL
	10 mM	0.3518 mL	1.7590 mL	3.5179 mL	8.7948 mL
	15 mM	0.2345 mL	1.1726 mL	2.3453 mL	5.8632 mL
	20 mM	0.1759 mL	0.8795 mL	1.7590 mL	4.3974 mL
	25 mM	0.1407 mL	0.7036 mL	1.4072 mL	3.5179 mL
	30 mM	0.1173 mL	0.5863 mL	1.1726 mL	2.9316 mL
	40 mM	0.0879 mL	0.4397 mL	0.8795 mL	2.1987 mL
	50 mM	0.0704 mL	0.3518 mL	0.7036 mL	1.7590 mL
	60 mM	0.0586 mL	0.2932 mL	0.5863 mL	1.4658 mL
	80 mM	0.0440 mL	0.2199 mL	0.4397 mL	1.0993 mL
	100 mM	0.0352 mL	0.1759 mL	0.3518 mL	0.8795 mL

Keywords

Calycosin

22 Cited Publications

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Publications Citing Use of MedChemExpress (MCE) Calycosin

Activité biologique

Chemical Information

Publications (22)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Calycosin Related Classifications

HY-N0519 Related Classifications