2. MAPK/ERK Pathway Stem Cell/Wnt PI3K/Akt/mTOR Apoptosis
  3. ERK JNK p38 MAPK PI3K Akt mTOR Apoptosis
  4. Ononin

Ononin  (Synonyms: Ononoside; Formononetin 7-O-β-D-glucopyranoside)

Cat. No.: HY-N0270 Purity: 99.97%
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Ononin is an orally active isoflavone. Ononin inhibits the ERK/JNK/p38 and PI3K/Akt/mTOR pathways. Ononin regulates Apoptosis. Ononin has anti-tumor effects on laryngeal cancer and lung cancer. Ononin has neuroprotective effects. Ononin alleviates endoplasmic reticulum stress and diabetic nephropathy.

For research use only. We do not sell to patients.

CAS No. : 486-62-4

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Customer Review

Based on 11 publication(s) in Google Scholar

Other Forms of Ononin:

Ononin Related Antibodies

Top Publications Citing Use of Products

    Ononin purchased from MedChemExpress. Usage Cited in: BMC Complement Med Ther. 2022 Jan 27;22(1):25.  [Abstract]

    Rat chondrocytes were intervened on Ononin (1 nM to 100 μM) only or with IL-1β (10 ng/mL) for 24 h and cell viability was detected by CCK-8 kit.

    Ononin purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2022 Oct 1:452:116179.  [Abstract]

    Ononin (30-60 mg/kg; oral gavage; once daily for 8 weeks) prevented Doxorubicin (DOX)-induced cardiac tissue damage in rats.

    Ononin purchased from MedChemExpress. Usage Cited in: BMC Complement Med Ther. 2022 Jan 27;22(1):25.  [Abstract]

    Expression levels of Collagen II and MMP-13 were detected by Western Blot. The samples derive from the same experiment and that different gels were processed in parallel. The results showed that IL-1β noticeably inhibited the expression of Collagen II and upregulated the expression of MMP-13. However, 10, 100, and 1000 nM Ononin (24 h) effectively reversed the degradation of Collagen II and the overexpression of MMP-13. Furthermore, Ononin ameliorated IL-1β-induced adverse effects on Collagen II and MMP-13 protein expression in a concentration-dependent manner.

    Ononin purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2022 Oct 1:452:116179.  [Abstract]

    CCK-8 assay data of H9C2 cells exposed to different concentrations of DOX and Ononin for 24 h. The results showed that 0.5 μM Ononin prevented the Doxorubicin (DOX)-induced reduction in cell viability.

    Ononin purchased from MedChemExpress. Usage Cited in: BMC Complement Med Ther. 2022 Jan 27;22(1):25.  [Abstract]

    Immunofluorescence staining of Collagen II protein (green), MMP-13 protein (red) and nucleus (blue) was labelled with DAPI and photographed by fluorescence microscope (20×) (scale bar: 50 μm). The results showed that Ononin (1000 nM; 24 h) decreased the expression of MMP-13 by approximately 2.9 times and Collagen II was restored to 90% compared with those in the IL-1β group, showing the ability to repair cartilage damage.

    Ononin purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2022 Oct 1:452:116179.  [Abstract]

    Ononin (0.1-1 μM; 24 h) pre-treatment prevented the Doxorubicin (DOX)-stimulated increase in the Bax/Bcl-2 ratio in H9C2 cells.

    View All ERK Isoform Specific Products:

    View All Isoforms
    ERK ERK1 ERK2 ERK5 ERK8

    View All JNK Isoform Specific Products:

    View All Isoforms
    JNK1 JNK2 JNK3 JNK

    View All p38 MAPK Isoform Specific Products:

    View All Isoforms
    p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

    View All PI3K Isoform Specific Products:

    View All Isoforms
    PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

    View All Akt Isoform Specific Products:

    View All Isoforms
    Akt Akt1 Akt2 Akt3

    View All mTOR Isoform Specific Products:

    View All Isoforms
    mTOR mTORC1 mTORC2

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Ononin is an orally active isoflavone. Ononin inhibits the ERK/JNK/p38 and PI3K/Akt/mTOR pathways. Ononin regulates Apoptosis. Ononin has anti-tumor effects on laryngeal cancer and lung cancer. Ononin has neuroprotective effects. Ononin alleviates endoplasmic reticulum stress and diabetic nephropathy[1][2][3][4][5][6].

    Cellular Effect
    Cell Line Type Value Description References
    HepG2 IC50
    40.8 μM
    Compound: 9
    Anticancer activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay
    Anticancer activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay
    		[PMID: 24974349]
    In Vitro

    Ononin (10-100 μM, 24 h) shows anticancer activity against laryngeal cancer Hep-2 cells via the inhibition of ERK/JNK/p38 signaling pathway[1].
    Ononin (6.25-100 μM, 48 h) shows anticancer activity against human HepG2 cells, with IC50 of 40.8 μM[2].
    Ononin (3-10  μM) exerts antitumor effects against lung cancer cells A549 and HCC827 by regulating apoptosis through inhibiting the PI3K/Akt/mTOR pathway[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Ononin Related Antibodies
    In Vivo

    Ononin (30 mg/kg, p.o., 12 days) shows neuroprotective effects against aluminium chloride-induced Alzheimer’s disease in rats[4].
    Ononin (10-50 mg/kg, p.o., once a day for 60 days) mitigates Streptozotocin (HY-13753)-induced diabetic nephropathy in rats via alleviating oxidative stress and inflammatory markers[5].
    Ononin (30-60 mg/kg/day, gavage, 8 weeks) alleviates endoplasmic reticulum stress in Doxorubicin (HY-15142A)-induced cardiotoxicity in rats by activating SIRT3[6].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    430.40

    Formula

    C22H22O9
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)[C@@H]1OC2=CC=C(C(C(C3=CC=C(OC)C=C3)=CO4)=O)C4=C2
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (232.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.3234 mL 11.6171 mL 23.2342 mL
    5 mM 0.4647 mL 2.3234 mL 4.6468 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (4.83 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (4.83 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.97%

    SDS (396 KB)

    COA (248 KB) HNMR (345 KB) CNMR (343 KB) RP-HPLC (234 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.3234 mL 11.6171 mL 23.2342 mL 58.0855 mL
    5 mM 0.4647 mL 2.3234 mL 4.6468 mL 11.6171 mL
    10 mM 0.2323 mL 1.1617 mL 2.3234 mL 5.8086 mL
    15 mM 0.1549 mL 0.7745 mL 1.5489 mL 3.8724 mL
    20 mM 0.1162 mL 0.5809 mL 1.1617 mL 2.9043 mL
    25 mM 0.0929 mL 0.4647 mL 0.9294 mL 2.3234 mL
    30 mM 0.0774 mL 0.3872 mL 0.7745 mL 1.9362 mL
    40 mM 0.0581 mL 0.2904 mL 0.5809 mL 1.4521 mL
    50 mM 0.0465 mL 0.2323 mL 0.4647 mL 1.1617 mL
    60 mM 0.0387 mL 0.1936 mL 0.3872 mL 0.9681 mL
    80 mM 0.0290 mL 0.1452 mL 0.2904 mL 0.7261 mL
    100 mM 0.0232 mL 0.1162 mL 0.2323 mL 0.5809 mL
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    Ononin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ononin486-62-4Ononoside Formononetin 7-O-β-D-glucopyranosideERKJNKp38 MAPKPI3KAktmTORApoptosisExtracellular signal regulated kinasesc-Jun N-terminal kinasePhosphoinositide 3-kinasePKBProtein kinase BMammalian target of RapamycinAnti-tumorNeuroprotectivearyngeal cancer and lung cancer.Inhibitorinhibitorinhibit

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