Cat. No.: HY-N0270

Ononin

(Synonyms: Ononoside; Formononetin 7-O-β-D-glucopyranoside)

11 Cited Publications

Customer Review

Based on 11 publication(s) in Google Scholar

Ononin is an orally active isoflavone. Ononin inhibits the ERK/JNK/p38 and PI3K/Akt/mTOR pathways. Ononin regulates Apoptosis. Ononin has anti-tumor effects on laryngeal cancer and lung cancer. Ononin has neuroprotective effects. Ononin alleviates endoplasmic reticulum stress and diabetic nephropathy.

For research use only. We do not sell to patients.

Purity: 99.97%
CAS No.: 486-62-4
Formula: C₂₂H₂₂O₉
Molecular Weight:430.40

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Ononin Chemical Structure

CAS No.: 486-62-4

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (11)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All ERK Isoforms :

All JNK Isoforms :

All p38 MAPK Isoforms :

All PI3K Isoforms :

All Akt Isoforms :

All mTOR Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	IC₅₀	40.8 μM Compound: 9	Anticancer activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay Anticancer activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 24974349]

In Vitro

Ononin (10-100 μM, 24 h) shows anticancer activity against laryngeal cancer Hep-2 cells via the inhibition of ERK/JNK/p38 signaling pathway^[1].
Ononin (6.25-100 μM, 48 h) shows anticancer activity against human HepG2 cells, with IC₅₀ of 40.8 μM^[2].
Ononin (3-10 μM) exerts antitumor effects against lung cancer cells A549 and HCC827 by regulating apoptosis through inhibiting the PI3K/Akt/mTOR pathway^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Ononin (30 mg/kg, p.o., 12 days) shows neuroprotective effects against aluminium chloride-induced Alzheimer’s disease in rats^[4].
Ononin (10-50 mg/kg, p.o., once a day for 60 days) mitigates Streptozotocin (HY-13753)-induced diabetic nephropathy in rats via alleviating oxidative stress and inflammatory markers^[5].
Ononin (30-60 mg/kg/day, gavage, 8 weeks) alleviates endoplasmic reticulum stress in Doxorubicin (HY-15142A)-induced cardiotoxicity in rats by activating SIRT3^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 486-62-4
Appearance Solid
Molecular Weight 430.40
Formula C₂₂H₂₂O₉
Color White to off-white
SMILES

O[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)[C@@H]1OC2=CC=C(C(C(C3=CC=C(OC)C=C3)=CO4)=O)C4=C2
Synonyms

Ononoside; Formononetin 7-O-β-D-glucopyranoside
Structure Classification
- Flavonoids
- Isoflavones
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (11)

All Histological Imaging/StainingCell Proliferation/Viability AssayWBIF

Sort by:

Journal Impact Factor

Acta Pharm Sin B

Chrysin serves as a novel inhibitor of DGK α/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). [Abstract]

2021 Jan;11(1):143-155. PMID: 33532186
Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530
Chin Med

Therapeutic potential of Sheng-Xian-Tang in doxorubicin-induced chronic heart failure by regulation of phenylalanine metabolism disruption. [Abstract]

2026 Jan 12;21(1):29. PMID: 41527105
Toxicol Appl Pharmacol

Ononin alleviates endoplasmic reticulum stress in doxorubicin-induced cardiotoxicity by activating SIRT3. [Abstract]

2022 Oct 1:452:116179. PMID: 35914558

Ononin purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2022 Oct 1:452:116179. [Abstract]

Ononin (30-60 mg/kg; oral gavage; once daily for 8 weeks) prevented Doxorubicin (DOX)-induced cardiac tissue damage in rats.

Ononin purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2022 Oct 1:452:116179. [Abstract]

CCK-8 assay data of H9C2 cells exposed to different concentrations of DOX and Ononin for 24 h. The results showed that 0.5 μM Ononin prevented the Doxorubicin (DOX)-induced reduction in cell viability.

Ononin purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2022 Oct 1:452:116179. [Abstract]

Ononin (0.1-1 μM; 24 h) pre-treatment prevented the Doxorubicin (DOX)-stimulated increase in the Bax/Bcl-2 ratio in H9C2 cells.
BMC Complement Med Ther

Ononin ameliorates inflammation and cartilage degradation in rat chondrocytes with IL-1β-induced osteoarthritis by downregulating the MAPK and NF-κB pathways. [Abstract]

2022 Jan 27;22(1):25. PMID: 35086536

Ononin purchased from MedChemExpress. Usage Cited in: BMC Complement Med Ther. 2022 Jan 27;22(1):25. [Abstract]

Rat chondrocytes were intervened on Ononin (1 nM to 100 μM) only or with IL-1β (10 ng/mL) for 24 h and cell viability was detected by CCK-8 kit.

Ononin purchased from MedChemExpress. Usage Cited in: BMC Complement Med Ther. 2022 Jan 27;22(1):25. [Abstract]

Expression levels of Collagen II and MMP-13 were detected by Western Blot. The samples derive from the same experiment and that different gels were processed in parallel. The results showed that IL-1β noticeably inhibited the expression of Collagen II and upregulated the expression of MMP-13. However, 10, 100, and 1000 nM Ononin (24 h) effectively reversed the degradation of Collagen II and the overexpression of MMP-13. Furthermore, Ononin ameliorated IL-1β-induced adverse effects on Collagen II and MMP-13 protein expression in a concentration-dependent manner.

Ononin purchased from MedChemExpress. Usage Cited in: BMC Complement Med Ther. 2022 Jan 27;22(1):25. [Abstract]

Immunofluorescence staining of Collagen II protein (green), MMP-13 protein (red) and nucleus (blue) was labelled with DAPI and photographed by fluorescence microscope (20×) (scale bar: 50 μm). The results showed that Ononin (1000 nM; 24 h) decreased the expression of MMP-13 by approximately 2.9 times and Collagen II was restored to 90% compared with those in the IL-1β group, showing the ability to repair cartilage damage.
Naunyn Schmiedebergs Arch Pharmacol

Ononin induces ferroptosis in colorectal cancer cells via the PI3K/AKT/Nrf2 pathway to enhance anti-cancer immunotherapy. [Abstract]

2025 Jul 8. PMID: 40627200
J Biochem Mol Toxicol

Ononin Inhibits the Growth and Metastasis of Hepatocellular Carcinoma by Suppressing MMP-2 and MMP-9 via PI3K/AKT/mTOR Signaling. [Abstract]

2026 Apr;40(4):e70804. PMID: 41878835
PLoS One

Ononin delays the development of osteoarthritis by down-regulating MAPK and NF-κB pathways in rat models. [Abstract]

2024 Oct 31;19(10):e0310293. PMID: 39480787
Exp Ther Med

Ononin alleviates H2O2-induced cardiomyocyte apoptosis and improves cardiac function by activating the AMPK/mTOR/autophagy pathway. [Abstract]

2021 Nov;22(5):1307. PMID: 34584565
Research Square Preprint

2021 Aug.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (232.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3234 mL

11.6171 mL

23.2342 mL

5 mM

0.4647 mL

2.3234 mL

4.6468 mL

10 mM

0.2323 mL

1.1617 mL

2.3234 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Ye B, et al. Ononin Shows Anticancer Activity Against Laryngeal Cancer via the Inhibition of ERK/JNK/p38 Signaling Pathway. Front Oncol. 2022 Jul 14;12:939646. [Content Brief]

[2]. Chen L, et al. Prediction of anti-tumor chemical probes of a traditional Chinese medicine formula by HPLC fingerprinting combined with molecular docking. Eur J Med Chem. 2014 Aug 18;83:294-306. [Content Brief]

[3]. Gong G, et al. Antitumor Effects of Ononin by Modulation of Apoptosis in Non-Small-Cell Lung Cancer through Inhibiting PI3K/Akt/mTOR Pathway. Oxid Med Cell Longev. 2022 Dec 27;2022:5122448. [Content Brief]

[4]. Chen X, et al. Neuroprotective effects of ononin against the aluminium chloride-induced Alzheimer's disease in rats. Saudi J Biol Sci. 2021 Aug;28(8):4232-4239. [Content Brief]

[5]. Dong L, et al. Ononin mitigates streptozotocin-induced diabetic nephropathy in rats via alleviating oxidative stress and inflammatory markers. Journal of King Saud University-Science, 2022, 34(6): 102029.

[6]. Zhang H, et al. Ononin alleviates endoplasmic reticulum stress in doxorubicin-induced cardiotoxicity by activating SIRT3. Toxicol Appl Pharmacol. 2022 Oct 1;452:116179. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3234 mL	11.6171 mL	23.2342 mL	58.0855 mL
	5 mM	0.4647 mL	2.3234 mL	4.6468 mL	11.6171 mL
	10 mM	0.2323 mL	1.1617 mL	2.3234 mL	5.8086 mL
	15 mM	0.1549 mL	0.7745 mL	1.5489 mL	3.8724 mL
	20 mM	0.1162 mL	0.5809 mL	1.1617 mL	2.9043 mL
	25 mM	0.0929 mL	0.4647 mL	0.9294 mL	2.3234 mL
	30 mM	0.0774 mL	0.3872 mL	0.7745 mL	1.9362 mL
	40 mM	0.0581 mL	0.2904 mL	0.5809 mL	1.4521 mL
	50 mM	0.0465 mL	0.2323 mL	0.4647 mL	1.1617 mL
	60 mM	0.0387 mL	0.1936 mL	0.3872 mL	0.9681 mL
	80 mM	0.0290 mL	0.1452 mL	0.2904 mL	0.7261 mL
	100 mM	0.0232 mL	0.1162 mL	0.2323 mL	0.5809 mL

Keywords

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Ononin

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

MAPK Compound Library

PI3K/Akt/mTOR Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Reprogramming Compound Library

Anti-diabetic Compound Library

Glycoside Compound Library

Oxygen Sensing Compound Library

Endoplasmic Reticulum Stress Compound Library

Medicine Food Homology Compound Library

Glycolysis Compound Library

Pyroptosis Compound Library

Cytoskeleton Compound Library

Orally Active Compound Library

Glutamine Metabolism Compound Library

Traditional Chinese Medicine Active Compound Library

Flavonoids Library

Anti-Alzheimer's Disease Compound Library

Neuroprotective Compound Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Transcription Factor-Targeted Library

Glucose Metabolism Compound Library

Food-Sourced Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Anti-Cancer Natural Product Library

Antidepressant Compound Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Pain-Related Compound Library

Mitochondrial Protection Compound Library

Membrane Protein-targeted Compound Library

Cell Death Library

Serine/Threonine Kinase Inhibitor Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Anti-Fibrosis Compound Library

Anti-Brain Cancer Compound Library

Miao Ethnicity Medicine Compound Library

Tibetan Medicine Compound Library

PBCRBS Traditional Chinese Medicine Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

RO5 Drug-like Natural Product Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Anti-Rheumatic Arthritis Compound Library