AlDeSense AM
AlDeSense AM is a cell-permeable ALDH1A1-selective fluorescent reporter. AlDeSense AM is oxidized by ALDH1A1, which eliminates photoinduced electron transfer quenching and enhances the fluorescent signal. AlDeSense AM can be used to detect cells with cancer stem cell properties, as well as to monitor the plasticity of cancer stem cells in cell culture systems and animal models. AlDeSense AM is applicable to the study of cancers associated with cancer stem cells, including chronic myeloid leukemia, melanoma, and breast cancer.
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- CAS No.: 2243148-62-9
- Formule: C24H16F2O6
- Masse moléculaire:438.38
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
AlDeSense AM (1.5-2 μM; 30 min) selectively labels human chronic myeloid leukemia K562 cells with active ALDH1A1, which correspond to the CD34+/CD38−/CD133+ leukemia stem cell population[1].
AlDeSense AM (2 μM; 1 h) detects elevated ALDH1A1 activity in MDA-MB-231 breast cancer mammospheres (cancer stem cell-enriched), and the fluorescent signal decreases as the mammospheres differentiate over 36 h[1].
The fluorescence signal of AlDeSense AM (2 μM; 1 h) in CSC-enriched B16F0 e-CSC is 11.3-fold higher than that in non-CSC B16F0 cells, confirming elevated ALDH1A1 activity in e-CSC[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:K562 human chronic myeloid leukemia cells
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Concentration:1.5 μM (flow cytometry staining); 2 μM (confocal imaging staining)
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Incubation Time:30 min at room temperature (confocal imaging staining)
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Result:Identified a population of brightly fluorescent cells corresponding to the CD34+/CD38-/CD133+ leukemic stem cell profile.
Showed a small population of highly fluorescent cells via confocal imaging.
Completely abolished the fluorescence signal after ALDH1A1 siRNA knockdown.
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Cell Line:MDA-MB-231 breast cancer mammospheres (CSC-enriched) and differentiating mammospheres
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Concentration:2 μM
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Incubation Time:1 h at room temperature (at 1, 6, 12, 24, 36 h time points during differentiation)
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Result:Exhibited 3-fold brighter fluorescence in freshly isolated mammospheres compared to control-stained mammospheres.
Gradually decreased fluorescence signal over 36 h of differentiation.
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Cell Line:B16F0 murine melanoma e-CSCs (CSC-enriched via spiral-patterned hydrogel culture) and non-CSCs (standard culture)
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Concentration:2 μM
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Incubation Time:1 h at room temperature
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Result:Showed 11.3-fold brighter fluorescence in e-CSCs than in non-CSCs.
Showed 9.0-fold brighter fluorescence in e-CSCs than in e-CSCs stained with control reagent.
Colocalized with the CD271 melanoma stem cell marker in e-CSCs.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6J (female, 6 to 8 weeks old)[1]
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Dosage:15 μM
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Administration:lung perfusion; single dose
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Result:Produced a mean fluorescence signal of ~1×1011 a.u.
in lungs with e-CSC metastases.
Showed a significantly higher mean fluorescence signal (~1×1011 a.u.) than the ~7×1010 a.u.
measured in lungs with non-CSC metastases.
Demonstrated significantly higher fluorescence in e-CSC metastases than e-CSC metastases treated with Ctrl-AlDeSense AM.
Chemical Information
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CAS No. 2243148-62-9
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Masse moléculaire 438.38
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Formule C24H16F2O6
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SMILES
CC(OCOC1=C(C=C2C(OC3=CC(C(F)=CC3=C2C4=CC=C(C=C4C)C=O)=O)=C1)F)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
AlDeSense AM Related Classifications
HY-D3185 Related Classifications
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- AlDeSense AM
- 2243148-62-9
- Fluorescent Dye
- Aldehyde Dehydrogenase (ALDH)
- melanoma
- breast cancer
- CD34+/CD38-/CD133+ leukemic stem cell population
- C57BL/6J mice
- chronic myeloid leukemia
- MDA-MB-231 breast cancer mammospheres
- cancer stem cells
- B16F0 e-CSCs
- K562 human chronic myeloid leukemia cells
- ALDH1A1
- Inhibitor
- inhibitor
- inhibit