Cy5 DBCO chloride
Cy5 DBCO chloride is an azide reaction probe and the addition of DBCO molecules allows the imaging of azide-labelled biomolecules by a copper-free "Click Chemistry" reaction. Cy5 DBCO (chloride) is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
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- CAS No.: 2182601-72-3
- Formule: C50H53ClN4O2
- Masse moléculaire:777.43
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
Cy5 DBCO chloride (2-50 μM) labels cells in a dose-dependent manner, and the amount of DBCO-Cy5 bound to the cell surface increases with increasing DBCO-Cy5 concentration[1].
Guide (The following is a recommended protocol for reference only and should be adjusted according to your specific experimental needs).
1. Seed the cells in a culture dish and culture for 3 days, then wash the cells twice with DPBS (pH 7.4).
2. Add Cy5 DBCO chloride at a final concentration of 20 μM and incubate at 37℃ for 1 h.
3. After incubation, wash the cells with DPBS (pH 7.4), then fix them with formaldehyde-glutaraldehyde combined fixative at room temperature for 15 min.
4. After fixation, wash the cells twice with DPBS (pH 7.4), and analyze the samples using a flow cytometer, fluorescence microscope, or fluorescence microplate reader.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Emission (Em)
668
Excitation (Ex)
647
Chemical Information
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CAS No. 2182601-72-3
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Masse moléculaire 777.43
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Formule C50H53ClN4O2
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SMILES
CC1(C)C(/C=C/C=C/C=C2N(CCCCCC(NCCC(N3C4=CC=CC=C4C#CC5=CC=CC=C5C3)=O)=O)C6=C(C=CC=C6)C/2(C)C)=[N+](C)C7=C1C=CC=C7.[Cl-]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Cy5 DBCO chloride Related Classifications
HY-D1625 Related Classifications
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