DWC 751
DWC 751 is a cephalosporin antibiotic. DWC 751 exhibits activity against most Gram-positive bacteria, such as methicillin-sensitive Staphylococcus aureus and S. pneumoniae, as well as Gram-negative bacteria including E. coli and E. cloacae. DWC 751 can be used in the research of infectious diseases.
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- CAS No.: 148098-42-4
- Formule: C21H22N8O9S3
- Masse moléculaire:626.64
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
Description
Chemical Information
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CAS No. 148098-42-4
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Masse moléculaire 626.64
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Formule C21H22N8O9S3
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SMILES
O=S(O)([O-])=O.OC(C1=C(CN2C3=C(C=CC=C3)[N+](C)=N2)CSC(C4NC(/C(C5=CSC(N)=N5)=N/OC)=O)N1C4=O)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)