Fast Red Violet LB
Fast Red Violet LB is a dye for staining tartrate resistant acid phosphatase (TRAP). Fast Red Violet LB can be used for alkaline phosphatase (ALP) activity staining.
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- CAS No.: 32348-81-5
- Formule: C14H11Cl2N3O
- Masse moléculaire:308.16
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Preparation of Dye Working Solution[2]
1.1 Prepare 0.1% Fast Red Violet LB solution.
2. Specific Staining Steps
2.1 Wash cells with PBS to remove residual reagents.
2.2 Fix cells with 4% paraformaldehyde for 10 min.
2.3 Wash with PBS to remove paraformaldehyde.
2.4 Incubate cells with Fast Red Violet LB working solution and 0.1% naphthol AS-MX phosphate at room temperature for 10 min.
2.5 Wash cells thoroughly with PBS to remove unbound dye.
2.6 Observe alkaline phosphatase (ALP)-positive cells under a microscope.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 32348-81-5
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Masse moléculaire 308.16
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Formule C14H11Cl2N3O
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SMILES
N#[N+]C1=CC(Cl)=C(NC(C2=CC=CC=C2)=O)C=C1C.[Cl-]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Mesenchymal stromal/stem cell osteogenic differentiation
Mesenchymal stromal/stem cells can be induced toward an osteoblast-like lineage in vitro by culture in osteogenic medium containing dexamethasone, ascorbic acid or ascorbate-2-phosphate, and β-glycerophosphate; the differentiation process is commonly evaluated by alkaline phosphatase activity, osteogenic marker expression, collagenous matrix formation, and calcium-rich matrix mineralization. The main readouts are alkaline phosphatase activity as an early osteogenic marker and Alizarin Red S staining as a calcium-deposit readout for mineralized extracellular matrix; Alizarin Red S can be inspected microscopically or extracted and measured colorimetrically at 405 nm.
Pureté et documentation
Références
[1]. Yan D, et al. Genetic background influences fluoride's effects on osteoclastogenesis. Bone. 2007 Dec;41(6):1036-44. [Content Brief]
[2]. Kotobuki N, et al. Cultured autologous human cells for hard tissue regeneration: preparation and characterization of mesenchymal stem cells from bone marrow. Artif Organs. 2004 Jan;28(1):33-9. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Fast Red Violet LB Related Classifications
HY-D1491 Related Classifications
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