PADI4_11

PADI4_11 binds selectively to calcium-bound and active-site-blocked calcium-bound recombinant hPADI4 with nanomolar affinity, but does not bind to calcium-free PADI4^[1].
PADI4_11 (0.003-100 μM) allosterically activates recombinant PADI4 by reducing its calcium ion requirement, enhances the maximum activity under calcium ion-saturated conditions, and exhibits isozyme selectivity for PADI4^[1].
PADI4_11 (100 nM-50 μM; 4 h) exhibits cell permeability in HaLo-GFP-mito HeLa cells, and its uptake is partially mediated by active transport mechanisms^[1].
PADI4_11 (1-50 μM; 1 h) specifically activates PADI4 in hPADI4-stable mouse embryonic stem cells (mES cells) without additional stimulation, shows no off-target effect on mouse PADI4, and exhibits no signs of cytotoxicity^[1].
PADI4_11 specifically binds to recombinant PADI4 in the calcium-bound active conformation, even when the active site is blocked, with a K_d value of 457 nM for the calcium-bound form^[2].
PADI4_11 (100 nM-50 μM; 4 h) exhibits cell permeability in HaLo-GFP-mito HeLa cells, with a CP₅₀ value of 3.1 μM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.