Calcein calcium potassium

Description: Calcein is a fluorescent probe with yellow green fluorescence, it can be used to determine labile iron pool and measure the Fe(III)-binding affinity with ligands^[3].
1. Solution preparation^[3]
1.1 Preparation of stock solution
Solvent: 1 M NaOH
Concentration: 0.5 mM (optimized according to the experiment).
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 0.5 µM with PBS or cell culture medium.
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. Cell staining
2.1 Cells (10⁶/mL) are loaded by incubation with 20 µM Fe(II) for 2 h at 37°C. 2.2 Cells are collected by centrifugation (1000 rpm; 5 min) and Calcein (0.5 µM) is directly injected into the cells via microinjection for staining (5-15 mins). 2.3 Wash cells for twice by D’hanks buffer (pH 7.4). 2.4 The fluorescence is monitored by both flow cytometry and a confocal laser scanning microscope.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.