CalFluor 647 azide

Guidelines (The recommended procedures below are for guidance only and should be adjusted according to your specific requirements)^[2]

1. Working Solution Preparation
1.1 Working concentration: 2 μM.
1.2 Precautions: Adjust the working solution concentration as needed; prepare fresh before use.

2. Staining Procedure
2.1 Sample type description: Solution samples (including CuCl₂ solution, artificial urine samples, bacterial suspension samples, and processed urine samples).
2.2 Incubation conditions: Use 2 μM CalFluor 647 azide working solution, incubate in the dark for 10 min with gentle shaking.

3. Detection and Analysis
3.1 Instrument type: Microplate reader.
3.2 Excitation/emission wavelengths: Excitation wavelength: 647 nm; emission wavelength: 650-800 nm.
3.3 Result analysis:
3.3.1 The higher the concentration of Cu²⁺ in the system, the stronger the fluorescence intensity.
3.3.2 In systems containing Cu²⁺, the higher the bacterial concentration, the weaker the fluorescence intensity.
3.3.3 After successful activation via click reaction, the dye emits red fluorescence.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.