Carbonic acid
Carbonic acid is a diprotic acid, acidulant and anesthetic. Carbonic acid penetrates living cells more easily than most other acids, exhibits stronger toxicity than inorganic acids at equivalent concentrations, and exacerbates ocean acidification. Carbonic acid binds reversibly to cellular proteins, acidifies cell sap and protoplasmic inclusions, inhibits Paramecium growth and various cellular functions, induces reversible cellular changes, produces lethal effects at high doses, decomposes into carbon dioxide and H2O, and participates in the maintenance of physiological pH balance.
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- CAS No.: 463-79-6
- Formula: CH2O3
- Molecular Weight:62.02
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Carbonic acid (0.03 Mol; until protoplasmic movement ceased) induces cessation of protoplasmic streaming in Tradescantia virginica anther hair cells at a mean intracellular pH of 4.5, equivalent to the cells' normal baseline pH[2].
Carbonic acid (0.03 Mol; 25 min-3 hours) induces reversible cessation of protoplasmic streaming in Tradescantia virginica anther hair cells, with full reversibility after exposures up to 1 hour and partial reversibility after 3 hours[2].
Carbonic acid (0.03 Mol; 1 hour, followed by 1 hour air exposure) exerts a reversible narcotic effect on Tradescantia virginica anther hair cells, with all cells resuming normal protoplasmic streaming after removal of the acid[2].
Carbonic acid is toxic to Paramaecium caudatum, causing complete loss of motility within 10 to 55 minutes depending on medium pH, with intracellular acidification of vacuoles preceding protoplasmic acidification and death[2].
Carbonic acid forms rapidly via photo-triggered deuteron transfer to bicarbonate in buffered D2O solution (pD=8) with an on-contact transfer time of 8 ps, has a deprotonation rate of 280,000 ps, and exhibits an acidity constant pKa of 3.45, with no observable decomposition to CO2 on the nanosecond timescale[3].
Carbonic acidforms upon rapid acidification, and decomposes to carbon dioxide with a measurable rate observable via continuous-flow Raman spectroscopy[3].
Carbonic acid forms in solid CO2-H2O ice mixtures after irradiation with 700 keV protons, high-flux UV radiation, or ion implantation, demonstrating relevance to astrophysical cryogenic environments[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Paramaecium caudatum[2]
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Dosage:Culture medium saturated with CO2
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Administration:continuous exposure via Lopriore-style chamber; up to 100 minutes
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Result:Reduced motile infusoria to 44% after 5 minutes and 0% after 10 minutes in medium pH 5.1 (Experiment 1).
Reduced motile infusoria to 44% after 5 minutes, 15% after 10 minutes, and 0% after 15 minutes in medium pH 5.05 (Experiment 2).
Reduced motile infusoria to 79% after 5 minutes, 48% after 10 minutes, 25% after 15 minutes, 19% after 20 minutes, 12% after 25 minutes, and 0% after 30 minutes in medium pH 5.1 (Experiment 3).
Reduced motile infusoria to 100% after 5 minutes, 37% after 10 minutes, 11% after 15 minutes, 4% after 20 minutes, and 0% after 25 minutes in medium pH 5.1 (Experiment 4).
Reduced motile infusoria to 100% after 5 minutes, 94% after 10 minutes, 82% after 15 minutes, 69% after 20 minutes, 16% after 30 minutes, 1% after 40 minutes, and 0% after 45 minutes in medium pH 5.2 (Experiment 5).
Reduced motile infusoria to 94% after 5 minutes, 79% after 10 minutes, 80% after 15 minutes, 55% after 20 minutes, 52% after 25 minutes, 40% after 30 minutes, 12% after 40 minutes, 1% after 50 minutes, and 0% after 55 minutes in medium pH 5.0 (Experiments 6 and 7).
Acidified cell vacuoles to pH ~4.9 (stained red) while protoplasm remained at pH ~6.0 (yellow); induced diffuse protoplasmic staining at pH ~5.5 immediately before death, accompanied by cell swelling and slowed movement.
Chemical Information
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CAS No. 463-79-6
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Molecular Weight 62.02
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Formula CH2O3
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SMILES
OC(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)