23491-45-4

Hoechst 33258 trihydrochloride Chemical Structure
23491-45-4

Chemical Structure

Hoechst 33258 trihydrochloride

Synonym(s): bisBenzimide H 33258 trihydrochloride; H 33258 trihydrochloride

  • CAS No.: 23491-45-4
  • Formula:C25H27Cl3N6O
  • Molecular Weight:533.88

IUPAC Name: 4-(6-(4-methylpiperazin-1-yl)-1H,3'H-[2,5'-bibenzo[d]imidazol]-2'-yl)phenol trihydrochloride

InChIKey: SMNPLAKEGAEPJD-UHFFFAOYSA-N

SMILES: OC1=CC=C(C2=NC3=CC=C(C4=NC5=CC=C(N6CCN(C)CC6)C=C5N4)C=C3N2)C=C1.[H]Cl.[H]Cl.[H]Cl

Biological Activity: Hoechst 33258 trihydrochloride is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution[1].

Cat. No. Product Name Purity Description Pricing
HY-15558A
Hoechst 33258 trihydrochloride 99.51% Hoechst 33258 trihydrochloride is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
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