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  2. para-iodoHoechst 33258

para-iodoHoechst 33258 

Cat. No.: HY-15632 Purity: 99.73%
Handling Instructions

para-iodoHoechst 33258 is part of a family of blue fluorescent dyes used to stain DNA.

For research use only. We do not sell to patients.

para-iodoHoechst 33258 Chemical Structure

para-iodoHoechst 33258 Chemical Structure

CAS No. : 158013-43-5

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 494 In-stock
Estimated Time of Arrival: December 31
5 mg USD 420 In-stock
Estimated Time of Arrival: December 31
10 mg USD 600 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1800 In-stock
Estimated Time of Arrival: December 31
100 mg USD 2520 In-stock
Estimated Time of Arrival: December 31
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Description

para-iodoHoechst 33258 is part of a family of blue fluorescent dyes used to stain DNA. Hoechst 33258 is a cell dye for DNA quantitation. IC50 Value: These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when protected from light. For long-term storage the solutions are instead frozen at ≤-20 °C. The dyes bind to the minor groove of double-stranded DNA with a preference for sequences rich in adenine andthymine. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably. Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells. Therefore, these stains are often called supravital, which means that cells survive a treatment with these compounds. Cells that express specific ATP-binding cassette transporter proteins can also actively transport these stains out of their cytoplasm.

Molecular Weight

534.39

Formula

C₂₅H₂₃IN₆

CAS No.

158013-43-5

SMILES

CN1CCN(C2=CC=C3N=C(C4=CC=C5N=C(C6=CC=C(I)C=C6)NC5=C4)NC3=C2)CC1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 20.5 mg/mL (38.36 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8713 mL 9.3565 mL 18.7129 mL
5 mM 0.3743 mL 1.8713 mL 3.7426 mL
10 mM 0.1871 mL 0.9356 mL 1.8713 mL
*Please refer to the solubility information to select the appropriate solvent.
References
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para-iodoHoechst 33258
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