31373-65-6
Chemical Structure
Hippuryl-His-Leu-OH
Synonym(s): N-Benzoyl-Gly-His-Leu
- CAS No.: 31373-65-6
- Formula:C21H27N5O5
- Molecular Weight:429.47
InChIKey: AAXWBCKQYLBQKY-IRXDYDNUSA-N
SMILES: CC(C)C[C@@H](C(O)=O)NC([C@@H](NC(CNC(C1=CC=CC=C1)=O)=O)CC2=CN=CN2)=O
Biological Activity: Hippuryl-His-Leu-OH (N-Benzoyl-Gly-His-Leu) is a specific substrate for angiotensin-converting enzyme (ACE I) and a molecular tool used for ACE activity detection in in vitro experiments. Hippuryl-His-Leu-OH is hydrolyzed by ACE through competitive binding. Under ACE catalysis, Hippuryl-His-Leu-OH undergoes hydrolysis to produce hippuric acid (HA). The amount of HA produced can be used to quantitatively assess ACE activity or screen for ACE inhibitors. The released His-Leu can also react with o-phthalaldehyde or Fluorescamine (HY-D0715) for fluorescence detection. Hippuryl-His-Leu-OH can be applied to the in vitro screening of ACE inhibitors for hypertension and cardiovascular diseases, and is also used in the study of ACE activity changes in physiological and pathological processes such as renal compensatory hypertrophy[1][2][3].
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Hippuryl-His-Leu-OH | Hippuryl-His-Leu-OH (N-Benzoyl-Gly-His-Leu) is a specific substrate for angiotensin-converting enzyme (ACE I) and a molecular tool used for ACE activity detection in in vitro experiments. Hippuryl-His-Leu-OH is hydrolyzed by ACE through competitive binding. Under ACE catalysis, Hippuryl-His-Leu-OH undergoes hydrolysis to produce hippuric acid (HA). The amount of HA produced can be used to quantitatively assess ACE activity or screen for ACE inhibitors. The released His-Leu can also react with o-phthalaldehyde or Fluorescamine (HY-D0715) for fluorescence detection. Hippuryl-His-Leu-OH can be applied to the in vitro screening of ACE inhibitors for hypertension and cardiovascular diseases, and is also used in the study of ACE activity changes in physiological and pathological processes such as renal compensatory hypertrophy. | |||||||||||||||||||||
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- [1]. Nishimura K, et al. Purification of angiotensin I-converting enzyme from human lung. Biochim Biophys Acta. 1977 Aug 11;483(2):398-408. [Content Brief]
- [2]. Santos RA, et al. An improved fluorometric assay of rat serum and plasma converting enzyme. Hypertension. 1985 Mar-Apr;7(2):244-52. [Content Brief]
- [3]. Horiuchi M, et al. Method for determination of angiotensin-converting enzyme activity in blood and tissue by high-performance liquid chromatography. J Chromatogr. 1982 Dec 10;233:123-30. [Content Brief]
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