906664-68-4
Chemical Structure
ATTO 647
- CAS No.: 906664-68-4
- Formula:C34H45ClN2O9S
- Molecular Weight:693.25
InChIKey: VYIRHRODYNBXRP-UHFFFAOYSA-N
SMILES: O=C(CCCN1C2=C(C(CC1(C)C)C)C=C(C=C3C=C4C(C=C3C5(C)C)=[N+](C(C)(C)C=C4CS(=O)(O)=O)CC)C5=C2)O.O=Cl(=O)([O-])=O
Biological Activity: ATTO 647 is a carborhodamine fluorophore and imaging tracer with photostable properties. ATTO 647 serves as a fluorescent probe to investigate cell membrane structure and diffusion characteristics. When conjugated with wheat germ agglutinin, ATTO 647 specifically binds to N-acetyl-β-D-glucosamine and sialic acid residues on membrane glycoproteins, enabling single-molecule tracing of glycoprotein diffusion. ATTO 647 exhibits highly stable fluorescence properties with significantly reduced blinking in mounting media such as ROXS (AA/MV) and ROXS (TX/TQ), whereas its brightness properties vary in Ibidi-MM and Vectashield. ATTO 647 can also be used to label histone H2B-GFP in fixed cells for confocal microscopy photobleaching experiments[1][2].
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ATTO 647 | ATTO 647 is a carborhodamine fluorophore and imaging tracer with photostable properties. ATTO 647 serves as a fluorescent probe to investigate cell membrane structure and diffusion characteristics. When conjugated with wheat germ agglutinin, ATTO 647 specifically binds to N-acetyl-β-D-glucosamine and sialic acid residues on membrane glycoproteins, enabling single-molecule tracing of glycoprotein diffusion. ATTO 647 exhibits highly stable fluorescence properties with significantly reduced blinking in mounting media such as ROXS (AA/MV) and ROXS (TX/TQ), whereas its brightness properties vary in Ibidi-MM and Vectashield. ATTO 647 can also be used to label histone H2B-GFP in fixed cells for confocal microscopy photobleaching experiments. | |||||||||||||||||||||
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References
- [1]. Bruckbauer A, et al. Nanopipette delivery of individual molecules to cellular compartments for single-molecule fluorescence tracking. Biophys J. 2007;93(9):3120-3131. [Content Brief]
- [2]. Cordes T, et al. Mechanisms and advancement of antifading agents for fluorescence microscopy and single-molecule spectroscopy. Phys Chem Chem Phys. 2011;13(14):6699-6709. [Content Brief]
