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Cat. No.: HY-188109

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Cas9-T2A-GFP mRNA (N1-Methylpseudo-UTP)-LNP

Cas9-T2A-GFP mRNA (N1-Methylpseudo-UTP)-LNP is a lipid nanoparticle (LNP) encapsulating Cas9-T2A-GFP mRNA (N1-Methylpseudo-UTP). Cas9-T2A-GFP mRNA (N1-Methylpseudo-UTP) encodes a Cas9 nuclease gene with two nuclear localization signals (NLS) and green fluorescent protein (GFP), which can be used for genome engineering experiments. N1-Methylpseudo-UTP can reduce the immunogenicity of the generated mRNA.

For research use only. We do not sell to patients.

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Please store the product under the recommended conditions in the Certificate of Analysis.

Cas9-T2A-GFP mRNA (N1-Methylpseudo-UTP)-LNP Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

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SMILES

[Cas9-T2A-GFP mRNA (N1-Methylpseudo-UTP)-LNP]
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Molecular Biology

Gene Editing
Gene editing modify specific sites within the genome through gene deletions, insertions or conversions to study functionally unknown genes or conduct gene therapy. It is also used to change the biological traits of organisms to establish new varieties. Gene editing techniques include zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas 9) (CRISPR/Cas9).
RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.

Purity & Documentation

Handling Instructions (2659 KB)

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Cas9-T2A-GFP mRNA (N1-Methylpseudo-UTP)-LNP

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Cas9-T2A-GFP mRNA (N1-Methylpseudo-UTP)-LNP

Biological Activity

Description

Chemical Information

Protocols

Purity & Documentation

Calculators

Help & FAQs

Cas9-T2A-GFP mRNA (N1-Methylpseudo-UTP)-LNP Related Classifications

HY-188109 Related Classifications

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