Cathepsin L/S-IN-1
Based on 1 Customer Validation
Cathepsin L/S-IN-1 is a dual inhibitor of Cathepsin L and Cathepsin S with IC50s of 4.10 μM and 1.79 μM, respectively. Cathepsin L/S-IN-1 shows a significant antimetastatic and invasive effects on pancreatic cancer BxPC-3 and PANC-1 cells.
For research use only. We do not sell to patients.
- Purity: 99.89%
- CAS No.: 3052930-88-5
- Formula: C29H33BrN4O4S
- Molecular Weight:613.57
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Cathepsin Isoforms
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Biological Activity
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cathepsin L 4.10 μM (IC50) |
cathepsin S 1.79 μM (IC50) |
Cathepsin L/S-IN-1 (compound B1a) (0-25 μM; 48 h) shows noncytotoxic agaisnt pancreatic cancer BxPC-3 and PANC-1 cells[1].
Cathepsin L/S-IN-1 (0-25 μM; 48 h) inhibits Cathepsin L and S activities without decreasing the mRNA level of them[1].
Cathepsin L/S-IN-1 (0-25 μM; 48 h) inhibits the migration and invasion of pancreatic cancer BxPC-3 and PANC-1 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:BxPC-3 and PANC-1 cells
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Concentration:0, 6.25, 12.5, and 25 μM
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Incubation Time:48 hours
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Result:Failed to suppress the viability of pancreatic cancer cells.
Chemical Information
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CAS No. 3052930-88-5
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Appearance Solid
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Molecular Weight 613.57
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Formula C29H33BrN4O4S
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Color White to off-white
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SMILES
O[C@@H](COC[C@H](NS(=O)(C1=CC=C(C=C1)Br)=O)CC2=CC=CC=C2)CN(CC3)CCN3C(C=CC=C4)=C4C#N
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (162.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.6298 mL | 8.1490 mL | 16.2981 mL | 40.7451 mL |
| 5 mM | 0.3260 mL | 1.6298 mL | 3.2596 mL | 8.1490 mL | |
| 10 mM | 0.1630 mL | 0.8149 mL | 1.6298 mL | 4.0745 mL | |
| 15 mM | 0.1087 mL | 0.5433 mL | 1.0865 mL | 2.7163 mL | |
| 20 mM | 0.0815 mL | 0.4075 mL | 0.8149 mL | 2.0373 mL | |
| 25 mM | 0.0652 mL | 0.3260 mL | 0.6519 mL | 1.6298 mL | |
| 30 mM | 0.0543 mL | 0.2716 mL | 0.5433 mL | 1.3582 mL | |
| 40 mM | 0.0407 mL | 0.2037 mL | 0.4075 mL | 1.0186 mL | |
| 50 mM | 0.0326 mL | 0.1630 mL | 0.3260 mL | 0.8149 mL | |
| 60 mM | 0.0272 mL | 0.1358 mL | 0.2716 mL | 0.6791 mL | |
| 80 mM | 0.0204 mL | 0.1019 mL | 0.2037 mL | 0.5093 mL | |
| 100 mM | 0.0163 mL | 0.0815 mL | 0.1630 mL | 0.4075 mL |
Cathepsin L/S-IN-1 Related Classifications
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