Anhydrous calcium sulfate (97%)
Based on 1 Customer Validation
Anhydrous calcium sulfate (97%) serves as an oil-based drilling fluid additive and an osteogenic material. Anhydrous calcium sulfate (97%) increases the plastic viscosity, yield point, apparent viscosity and gel strength of oil-based drilling fluids. Anhydrous calcium sulfate (97%) upregulates the expression of bone-related genes FOSL1, RUNX2 and SPP1. Anhydrous calcium sulfate (97%) significantly affects the behavior of dental pulp stem cells, enhancing their proliferation, differentiation and matrix deposition.
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- Reinheit : 99.40%
- CAS. Nr.: 7778-18-9
- Formel: CaSO4
- Molecular Weight:136.14
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Speicherung:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biologische Aktivität
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Chemical Information
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Help & FAQs
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Biologische Aktivität
Beschreibung
IC50 & Target
[1]|
RUNX2 |
In Vitro
Anhydrous calcium sulfate (0.001 mg/mL; 30 days) upregulates the expression of bone-related genes in DPSC cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:DPSCs
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Concentration:0.001 mg/mL
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Incubation Time:30 days
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Result:Upregulated FOSL1, RUNX2, and SPP1 expression, while downregulating SP7, ENG, COL3A1, COL1A1, and ALPL expression.
Chemical Information
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CAS. Nr. 7778-18-9
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Appearance Solid
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Molecular Weight 136.14
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Formel CaSO4
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Color White to off-white
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SMILES
[Ca]=S(O[O])([O])=O
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Synonyms
Gypsum ustum (97%)
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Protokoll
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Reinheit & Dokumentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Murtaza M, et al. Application of Anhydrous Calcium Sulfate as a Weighting Agent in Oil-Based Drilling Fluids. ACS Omega. 2021;6(33):21690-21701. Published 2021 Aug 11. [Content Brief]
[2]. Sollazzo V, et al. Calcium sulfate stimulates pulp stem cells towards osteoblasts differentiation. Int J Immunopathol Pharmacol. 2011;24(2 Suppl):51-57. [Content Brief]
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
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