Caraphenol A
Based on 1 Customer Validation
Caraphenol A is a resveratrol trimer and is able to transiently reduce interferon-induced transmembrane (IFITM) protein expression. Caraphenol A safely enhances lentiviral vector gene delivery to hematopoietic stem and progenitor cells. Caraphenol A also inhibits human cystathionine β-synthase (hCBS) and human cystathionine γ- lyase (hCSE) with IC50s of 5.9 μM and 12.1 μM, respectively.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.71%
- CAS. Nr.: 354553-35-8
- Formel: C42H28O9
- Molecular Weight:676.67
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Speicherung:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
IC50 & Target
In Vitro
Caraphenol A (0-50 μM; 8 h) enhances lentiviral vector (LV) gene delivery to HeLa, but not HEK293T cells[1].
Caraphenol A decreases interferon induced transmembrane protein-mediated restriction in an endosomal trafficking dependent manner[1].
Caraphenol A treatment significantly improves hematopoietic stem cell (HSC) gene delivery at both low and high LV doses without altering LV integration patterns[1].
Caraphenol A (30 μM; 6 h) treatment facilitates lentiviral escape from endosomes[1].
Caraphenol A (30 μM; 4 h) alters expression and subcellular localization of IFITM2/3 protein and late endosomes in HeLa cells. The effect on late endosome is dependent on IFITM3 expression[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 354553-35-8
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Appearance Solid
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Molecular Weight 676.67
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Formel C42H28O9
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Color Light yellow to yellow
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SMILES
[H][C@]12[C@@H](OC3=C1C([C@@]4([C@H](OC5=C4C(C6=C(OC7=C6C2=CC(O)=C7)C8=CC=C(O)C=C8)=CC(O)=C5)C9=CC=C(O)C=C9)[H])=CC(O)=C3)C%10=CC=C(O)C=C%10
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Structure Classification
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 50 mg/mL (73.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Protokoll
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Western Blot
Western blotting (WB) is a commonly used experimental method in molecular biology, biochemistry, and immunogenetics for identifying and quantifying target proteins. It combines gel electrophoresis with immunoassay, enabling researchers to analyze protein expression, post-translational modifications, and molecular weight.
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Protocol For Protein Expression And Purification
Recombinant protein expression in Escherichia coli followed by purification of a His-tagged soluble protein by immobilized metal affinity chromatography (IMAC), with optional MBP fusion and TEV tag removal when the construct includes these elements. The biological readout is production of the encoded target protein, detected as an inducible band at the expected molecular mass by SDS-PAGE and quantified by total protein assay or chromatographic absorbance; the purification readout is enrichment of the target protein in elution fractions after selective binding of polyhistidine residues to immobilized Ni2+/metal-chelate resin and elution by imidazole-containing buffer. Expression is driven by an inducible bacterial expression system, commonly T7/lac-based, in which IPTG or lactose/auto-induction activates transcription and translation of the cloned gene; lower induction temperature, lower inducer concentration, induction timing, and solubility-enhancing fusion tags can influence the frac
Reinheit & Dokumentation
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Data Sheet (283 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Ozog S, et al. Resveratrol trimer enhances gene delivery to hematopoietic stem cells by reducing antiviral restriction at endosomes. Blood. 2019 Oct 17;134(16):1298-1311. [Content Brief]
[2]. Niu W, et al. Discovery of selective cystathionine β-synthase inhibitors by high-throughput screening with a fluorescent thiol probe. Medchemcomm. 2016 Nov 15;8(1):198-201. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4778 mL | 7.3891 mL | 14.7783 mL | 36.9456 mL |
| 5 mM | 0.2956 mL | 1.4778 mL | 2.9557 mL | 7.3891 mL | |
| 10 mM | 0.1478 mL | 0.7389 mL | 1.4778 mL | 3.6946 mL | |
| 15 mM | 0.0985 mL | 0.4926 mL | 0.9852 mL | 2.4630 mL | |
| 20 mM | 0.0739 mL | 0.3695 mL | 0.7389 mL | 1.8473 mL | |
| 25 mM | 0.0591 mL | 0.2956 mL | 0.5911 mL | 1.4778 mL | |
| 30 mM | 0.0493 mL | 0.2463 mL | 0.4926 mL | 1.2315 mL | |
| 40 mM | 0.0369 mL | 0.1847 mL | 0.3695 mL | 0.9236 mL | |
| 50 mM | 0.0296 mL | 0.1478 mL | 0.2956 mL | 0.7389 mL | |
| 60 mM | 0.0246 mL | 0.1232 mL | 0.2463 mL | 0.6158 mL |
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