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Art. -Nr.: HY-P3208

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Endoproteinase Lys-C

2 Cited Publications

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Based on 2 publication(s) in Google Scholar

Endoproteinase Lys-C is a protease that cleaves proteins on the C-terminal side of lysine residues and is commonly used for protein sequencing.

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CAS. Nr.: 72561-05-8

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Endoproteinase Lys-C Chemical Structure

CAS. Nr.: 72561-05-8

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
- EC Number
- Enzyme Activity
- Unit Definition
Chemical Information
Publications (2)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Endoproteinase Lys-C is a protease that cleaves proteins on the C-terminal side of lysine residues and is commonly used for protein sequencing^[1].

In Vitro

This product can be used for: (1) protein sequence analysis; (2) synthesis of lysine-related derivatives.

Protocol
This product can be directly used for protein enzyme digestion. The recommended enzyme digestion ratio is 1:50-1:200 (W/W), and the enzyme digestion should be performed at 37 °C overnight.

Notes
( 1) The optimal reaction pH of this product is 9.0-9.5, and the optimal reaction temperature is 30-37 °C. It can still maintain high enzyme activity in the presence of 4 M urea or 0.1% SDS.
( 2) Storage Buffer: 20 mM Tris-HCl, pH=8.0.
( 3) Do not freeze and thaw repeatedly.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EC Number

3.4.21.50

Enzyme Activity

≥1 AU/mg soild

Unit Definition

One activity unit is defined as the amount of enzyme that can catalyzes the substrate to produce 1 μ mol of p-Nitroaniline per minute at 30°C and pH 9.5.

Chemical Information

CAS. Nr. 72561-05-8
Appearance Solid
Color White to off-white
SMILES

[Endoproteinase Lys-C]
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (2)

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Cell Syst

Identifying microbial protease allergens through protein language model-guided homology. [Abstract]

2026 Feb 20:101510. PMID: 41722567
Adv Biol (Weinh)

A Novel UBM/SIS Composite Biological Scaffold for 2-Year Abdominal Defect Repairing and Strength Recovery in Canine Model. [Abstract]

2025 Jan;9(1):e2400131. PMID: 39542874

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : ≥ 50 mg/mL

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (268 KB)
SDS (558 KB)
- English - EN (558 KB)
- Français - FR (558 KB)
- Deutsch - DE (558 KB)
- Norwegian - NO (558 KB)
- Español - ES (558 KB)
- Swedish - SV (558 KB)
- Italian - IT (558 KB)
- Korean - KR (558 KB)
- Portuguese - PT (558 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. P A Jekel, et al. Use of endoproteinase Lys-C from Lysobacter enzymogenes in protein sequence analysis. Anal Biochem. 1983 Oct 15;134(2):347-54. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Endoproteinase Lys-C

2 Cited Publications

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Biologische Aktivität
- Beschreibung
- In Vitro
- EC Number
- Enzyme Activity
- Unit Definition
Chemical Information
Publications (2)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise

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Endoproteinase Lys-C

Publications Citing Use of MedChemExpress (MCE) Endoproteinase Lys-C

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Calculators

Help & FAQs

Endoproteinase Lys-C Related Classifications

HY-P3208 Related Classifications