o-Pah

o-Pah (20 μM) shows changed colour upon addition of 100 μM different metal ions and the further addition of 20 μM of Cu²⁺ in DMSO/HEPES buffer solution^[1].
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Procedures of metal ion sensing Stock solutions of the metal ions (100 mM and 10 mM) were prepared in deionised water^[1].
1. A stock solution of o-Pah (0.5 mM) is prepared in DMSO.
2. The solution of o-Pah is then diluted to 5 μM with HEPES buffer solution (50 mM KNO₃, 50 mM HEPRS, pH 7.2).
3. During titration experiments, 2 mL solutions of o-Pah (5 μM, 0.5 μM) are placed in a 1 cm quartz optical cell and Cu²⁺, Hg²⁺ stock solutions are added gradually with a micropipette.
4. In selectivity experiments, the test samples are prepared by adding appropriate amounts of the metal ion stock solution into the 2 mL solution of o-Pah (5 μM).
6. During fluorescence measurements, the excitation wavelength is 483 nm and emission spectra are collected between 495-600 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.