2,4,6-Trihydroxybenzaldehyde, a potential anti-obesity treatment, suppressed adipocyte differentiation in 3T3-L1 cells and fat accumulation induced by high-fat diet in C57BL/6 mice

  • Environ Toxicol Pharmacol. 2015 Mar;39(2):962-8. doi: 10.1016/j.etap.2015.02.007.
Kil-Nam Kim  1 ,  Min-Cheol Kang  2 ,  Nalae Kang  2 ,  Seo-Young Kim  2 ,  Chang-Gu Hyun  3 ,  Seong Woon Roh  1 ,  Eun-Yi Ko  4 ,  Kichul Cho  1 ,  Won-Kyo Jung  5 ,  Ginnae Ahn  6 ,  You-Jin Jeon  7 ,  Daekyung Kim  8
Affiliations
  • 1. Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea.
  • 2. School of Marine Biomedical Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
  • 3. Cosmetic Sciences Center, Faculty of Chemistry and Cosmetics, Jeju National University, Jeju 690-756, Republic of Korea.
  • 4. Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea; School of Marine Biomedical Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
  • 5. Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan 608-737, Republic of Korea.
  • 6. Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 550-749, Republic of Korea.
  • 7. School of Marine Biomedical Sciences, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: [email protected].
  • 8. Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea. Electronic address: [email protected].
PMID: 25812771 DOI: 10.1016/j.etap.2015.02.007
Abstract

In the present study, 2,4,6-trihydroxybenzaldehyde (THB) was evaluated for inhibitory effects on adipocyte differentiation in 3T3-L1 cells and anti-obesity effects in mice with high-fat diet (HFD)-induced obesity. Lipid accumulation measurement indicated that THB markedly inhibited adipogenesis, and this involved down-regulation of the expression of the adipogenesis-related proteins, CCAAT/enhancer-binding protein α (C/EBPα), Peroxisome Proliferator-activated Receptor γ (PPARγ), fatty acid synthase (FAS) and sterol regulatory element-binding protein-1c (SREBP-1c), in 3T3-L1 pre-adipocyte cells. In a mouse model of HFD-induced obesity, oral administration of THB (5 and 25mg/kg for 13 weeks) reduced the HFD-induced increase in weight gain. THB administration also reduced serum levels of glucose, triglycerides, and total Cholesterol. A reduction in the hypertrophy of white adipose tissue was also observed. Furthermore, THB administration inhibited HFD-induced hepatic steatosis. These results provided evidence that administration of THB alleviated HFD-induced obesity in C57BL/6 mice and revealed the potential of THB as a nutraceutical to help prevent or treat obesity and the associated metabolic disorders.

Keywords
2,4,6-Trihydroxybenzaldehyde (THB); 3T3-L1 cells; Anti-obesity; C57BL/6 mice; High fat diet (HFD).
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