country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

Cat. No.: HY-151542

Copy Link Print Information

MLKL-IN-4

MLKL-IN-4 (compound 56) is a potent MLKL (Mixed lineage kinase domain-like protein) inhibitor. MLKL-IN-4 inhibits necroptosis in HT-29 cells and acts downstream of MLKL phosphorylation, with EC₅₀ of 82 nM. MLKL-IN-4 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.

CAS No.: 3031406-17-1
Formule: C₃₀H₂₇ClN₄O₅
Masse moléculaire:559.01

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (265 KB)

MLKL-IN-4 Chemical Structure

CAS No.: 3031406-17-1

Size

Prix

Stock

Quantité

Amount: USD 0.00

* Veuillez sélectionner la quantité avant d'ajouter des articles.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

MLKL-IN-4 (compound 56) is a potent MLKL (Mixed lineage kinase domain-like protein) inhibitor. MLKL-IN-4 inhibits necroptosis in HT-29 cells and acts downstream of MLKL phosphorylation, with EC₅₀ of 82 nM^[1]. MLKL-IN-4 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

Voir tous les produits spécifiques à Isoform RIP kinase :

Cellular Effect

Cell Line	Type	Value	Description	References
HT-29	EC₅₀	>10 μM Compound: 56	Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 5 min followed by compound washout and further induced with TSZ by celltiter-glo luminescent cell viability assay Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 5 min followed by compound washout and further induced with TSZ by celltiter-glo luminescent cell viability assay	[PMID: 36136378]
HT-29	EC₅₀	180.3 nM Compound: 56	Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 3 hr followed by compound washout and further induced with TSZ by celltiter-glo luminescent cell viability assay Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 3 hr followed by compound washout and further induced with TSZ by celltiter-glo luminescent cell viability assay	[PMID: 36136378]
HT-29	EC₅₀	188.1 nM Compound: 56	Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 3 hrs followed by TSZ stimulation by celltiter-glo luminescent cell viability assay Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 3 hrs followed by TSZ stimulation by celltiter-glo luminescent cell viability assay	[PMID: 36136378]
HT-29	EC₅₀	584.3 nM Compound: 56	Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 1 hr followed by compound washout and further induced with TSZ by celltiter-glo luminescent cell viability assay Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 1 hr followed by compound washout and further induced with TSZ by celltiter-glo luminescent cell viability assay	[PMID: 36136378]
HT-29	EC₅₀	82 nM Compound: 56	Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ (TNFalpha, Smac mimetic and z-VAD-FMK) induced necroptosis incubated for 24 hrs by celltiter-glo luminescent cell viability assay Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ (TNFalpha, Smac mimetic and z-VAD-FMK) induced necroptosis incubated for 24 hrs by celltiter-glo luminescent cell viability assay	[PMID: 36136378]

In Vitro

MLKL-IN-4 (compound 56) (10 μM) slightly inhibits RIPK1, does not affect the phosphorylation status of RIPK1 and MLKL^[1].
MLKL-IN-4 (1 μM, 24 h) inhibits the translocation of MLKL to cell membranes in HT-29 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

MLKL-IN-4 (compound 56) shows T1/2 over 48 h and more than 150-fold lower reaction rates with glutathione (GSH), which potentially decreased their off-target effects and cell toxicity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 3031406-17-1
Masse moléculaire 559.01
Formule C₃₀H₂₇ClN₄O₅
SMILES

O=C(NC1=C(Cl)N(CC#CC2=CC=C(O)C(CN3C=CC=CC3=O)=C2)C(N(C)C1=O)=O)CCC4=CC=C(C)C=C4
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (265 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Cui B, et al. Discovery of a New Class of Uracil Derivatives as Potential Mixed Lineage Kinase Domain-like Protein (MLKL) Inhibitors. J Med Chem. 2022 Oct 13;65(19):12747-12780. [Content Brief]

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

MOQ

Minimum order quantity

100 mg

Obtenir un devis En stock

Other size

Obtenir un devis

Amount: USD 0.00

Activité biologique
- Description
- In Vitro
- In Vivo
Chemical Information
Pureté et documentation
Références

Nom du produit:
Cat. No.:
Quantité:
MCE Japan Authorized Agent:

Nom du produit:
CAS No.:
Species:
Pureté:
Quantité:

MLKL-IN-4

Voir tous les produits spécifiques à Isoform RIP kinase

Activité biologique

Chemical Information

Pureté et documentation

Références

Calculators

Help & FAQs

MLKL-IN-4 Related Classifications

HY-151542 Related Classifications