PE-Cy5
PE-Cy5 is a tandem fluorescent dye commonly used in flow cytometry, immunofluorescence, and cell biology research. It is formed by the covalent linkage of two fluorescent molecules, namely phycoerythrin and CY5. PE-Cy5 binds to human FcγRI (CD64), and this binding can be blocked by human pooled serum, anti-CD64 monoclonal antibodies targeting the ligand-binding region, or aggregated IgG. PE-Cy5 supports three-color flow cytometry analysis, and whole blood staining can partially reduce its non-specific binding (Ex/Em = 450-500 nm/665 nm).
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
PE-Cy5 conjugate specifically binds to cells expressing human FcγRI/CD64, including primary human monocytes, G-CSF-treated neutrophils, IFNγ-stimulated U937 cells, and FcγRI-transfected IIA1.6 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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SMILES
[PE-Cy5]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
[1]. van Vugt MJ, van den Herik-Oudijk IE, et al. Binding of PE-CY5 conjugates to the human high-affinity receptor for IgG (CD64). Blood. 1996 Sep 15;88(6):2358-61. [Content Brief]
[2]. Hulspas R, et al. Flow cytometry and the stability of phycoerythrin-tandem dye conjugates. Cytometry A. 2009;75(11):966-972. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PE-Cy5 Related Classifications
HY-D3274 Related Classifications
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- PE-Cy5
- Fluorescent Dye
- acute myeloid leukemia-fab m3
- anti-CD64 monoclonal antibodies
- G-CSF-treated patient neutrophils
- IFNγ-stimulated U937 cells
- primary human monocytes
- human pooled serum
- aggregated IgG
- human peripheral blood mononuclear cells
- human FcγRI (CD64)
- FcγRI-transfected IIA1.6 cells
- Inhibitor
- inhibitor
- inhibit