CMV pp65 (123-131)
CMV pp65 (123-131) is an HLA-B35:01-restricted CD8+ T cell epitope peptide derived from cytomegalovirus pp65, with the sequence IPSINVHHY. CMV pp65 (123-131) is applicable to studies on CMV-specific T cell detection, TCR cross-reactivity, and transplantation immunology.
For research use only. We do not sell to patients.
- CAS No.: 151868-77-8
- Formula: C50H74N14O13
- Molecular Weight:1079.21
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
CMV pp65 (123-131) HLA-B*35:01/IPSINVHHY-specific CD8+ T cells (CFSE 5 μM; IL-2 10 U/mL; stimulated with allogeneic PBMCs; 8 days) exhibit proliferative responses to HLA-B51+ and/or HLA-B58+ allogeneic cells in 23% (3/13) of tetramer-positive healthy donors, with HLA-B51-induced proliferation being stronger than that induced by HLA-B58[1].
CMV pp65 (123-131) HLA-B*35:01/IPSINVHHY-specific CD8+ T cell clones (E:T ratios of 30:1, 10:1, 1:1, and 0.1:1; IL-2 10 U/mL; 4 h) lyse HLA-B51+ PHA blasts, EBV-LCLs, PTECs, and HUVECs, whereas HLA-B58/B57 cross-reactive clones cannot effectively lyse the corresponding tested target cells[1].
CMV pp65 (123-131)/HLA-B*35:01 (IPSINVHHY)-specific CD8+ memory T cell clones exhibit shared allo-HLA cross-reactivity with highly conserved usage of TCR Vα and Vβ. Only minor CDR3β amino acid differences exist among distinct clones, and these differences can affect the cross-recognition patterns of HLA-B51 versus HLA-B58/B57[1].
CMV pp65 (123-131) HLA-B*35:01/IPSINVHHY-specific CD8+ T cell clones (5 × 103 T cells; 5 × 104 EBV-LCLs; IL-2 10 U/mL; 24 h) produce IFN-γ in response to HLA-B51+, HLA-B58+ and HLA-B57+ EBV-LCLs, with stronger responses induced by HLA-B51 and HLA-B58 than by HLA-B57[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:CMV pp65 (123-131)-specific CD8+ T cell clones; 51Cr-labeled PHA blasts, EBV-LCLs, PTECs, and HUVECs
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Concentration:10 U/mL (IL-2, 4-hour incubation)
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Incubation Time:4 h (with target cells)
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Result:HLA-B51-cross-reactive CMV pp65 (123-131)-specific T cell clones lysed HLA-B51+ PHA blasts, EBV-LCLs, PTECs, and HUVECs, with allo-HLA responses corresponding to 49%-97%, 44%-83%, 87%-104%, and 76%-98% of the cognate antiviral responses, respectively.
HLA-B58/B57-cross-reactive clones did not efficiently lyse the tested HLA-B58/B57+ target cells; HLA-B58+ HUVECs were not tested.
Chemical Information
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CAS No. 151868-77-8
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Molecular Weight 1079.21
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Formula C50H74N14O13
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SMILES
O=C(N1[C@@H](CCC1)C(N[C@@H](CO)C(N[C@@H]([C@@H](C)CC)C(N[C@@H](CC(N)=O)C(N[C@@H](C(C)C)C(N[C@@H](CC2=CNC=N2)C(N[C@@H](CC3=CNC=N3)C(N[C@@H](CC4=CC=C(C=C4)O)C(O)=O)=O)=O)=O)=O)=O)=O)=O)[C@H]([C@@H](C)CC)N
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Sequence
Ile-Pro-Ser-Ile-Asn-Val-His-His-Tyr
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Sequence Shortening
IPSINVHHY
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. van den Heuvel H, et al. Allo-HLA Cross-Reactivities of Cytomegalovirus-, Influenza-, and Varicella Zoster Virus-Specific Memory T Cells Are Shared by Different Healthy Individuals. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 2017 Aug;17(8):2033-2044. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
CMV pp65 (123-131) Related Classifications
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