Coumarin boronic acid
Based on 1 Customer Validation
Coumarin boronic acid is a fluorescent probe. The excitation and emission wavelengths of coumarin boronic acid are set to 360 nm and 430 nm, respectively. Coumarin boronic acid can be used to monitor the formation of amino acid and protein hydroxyl peroxides in real time, which is beneficial for understanding the mechanisms of oxidative stress and protein post-translational modification.
For research use only. We do not sell to patients.
- Purity : 99.93%
- CAS No.: 1357078-03-5
- Formula: C9H7BO4
- Molecular Weight:189.96
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
Chemical Information
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CAS No. 1357078-03-5
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Appearance Solid
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Molecular Weight 189.96
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Formula C9H7BO4
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Color White to off-white
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SMILES
O=C(O1)C=CC2=C1C=C(B(O)O)C=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
In Vitro:
DMSO : 83.33 mg/mL (438.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
Purity & Documentation
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Data Sheet (270 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.2643 mL | 26.3213 mL | 52.6427 mL | 131.6066 mL |
| 5 mM | 1.0529 mL | 5.2643 mL | 10.5285 mL | 26.3213 mL | |
| 10 mM | 0.5264 mL | 2.6321 mL | 5.2643 mL | 13.1607 mL | |
| 15 mM | 0.3510 mL | 1.7548 mL | 3.5095 mL | 8.7738 mL | |
| 20 mM | 0.2632 mL | 1.3161 mL | 2.6321 mL | 6.5803 mL | |
| 25 mM | 0.2106 mL | 1.0529 mL | 2.1057 mL | 5.2643 mL | |
| 30 mM | 0.1755 mL | 0.8774 mL | 1.7548 mL | 4.3869 mL | |
| 40 mM | 0.1316 mL | 0.6580 mL | 1.3161 mL | 3.2902 mL | |
| 50 mM | 0.1053 mL | 0.5264 mL | 1.0529 mL | 2.6321 mL | |
| 60 mM | 0.0877 mL | 0.4387 mL | 0.8774 mL | 2.1934 mL | |
| 80 mM | 0.0658 mL | 0.3290 mL | 0.6580 mL | 1.6451 mL | |
| 100 mM | 0.0526 mL | 0.2632 mL | 0.5264 mL | 1.3161 mL |
Coumarin boronic acid Related Classifications
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