Cusculine
Based on 1 Customer Validation
Cusculine (FAC21) is an oropouche virus (OROV) endonuclease inhibitor with an IC50 of 1.4 μM. Cusculine strongly interacts with OROV endonuclease, possibly limiting viral RNA interaction with other proteins, intercalates into double-stranded RNA (a viral replication intermediate), and efficiently inhibits OROV viral replication in vitro. Cusculine can be used for the research of oropouche fever.
For research use only. We do not sell to patients.
- Purity: 99.76%
- CAS No.: 143438-95-3
- Formula: C18H19NO6
- Molecular Weight:345.35
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HeLa | IC50 |
8.5 μg/mL
Compound: 2, Cusculine
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Cytotoxicity against human HeLa cells by MTT assay
Cytotoxicity against human HeLa cells by MTT assay
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[PMID: 1431935] |
Cusculine (FAC21) (72 h) exhibits low cytotoxicity in Vero cells (CC50 = 1640 μM)[1].
Cusculine (15.125-250 μM) potently inhibits OROV infection in Vero cells (EC50 = 33.03 μM)[1].
Cusculine (125 μM; 8-48 hpi) strongly inhibits OROV replication early in Vero cells (99.9% inhibition at 8 hpi)[1].
Cusculine (18.5 μM; 45 min) modestly interacts with dsRNA, showing partial intercalation activity[1].
Cusculine (0-90 μM; 10 min) potently inhibits OROV N-terminal endonuclease as a competitive inhibitor (IC50 = 1.4 μM)[1].
Cusculine stably binds to the active site of OROV N-terminal endonuclease, with favorable binding free energy and conserved interacting residues[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 143438-95-3
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Appearance Solid
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Molecular Weight 345.35
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Formula C18H19NO6
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Color Light yellow to yellow
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SMILES
O=C1C2=CC=C(OC)C(OC)=C2NC3=CC(OC)=C(OC)C(OC)=C13
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Synonyms
FAC21
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 41.67 mg/mL (120.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8956 mL | 14.4781 mL | 28.9561 mL | 72.3903 mL |
| 5 mM | 0.5791 mL | 2.8956 mL | 5.7912 mL | 14.4781 mL | |
| 10 mM | 0.2896 mL | 1.4478 mL | 2.8956 mL | 7.2390 mL | |
| 15 mM | 0.1930 mL | 0.9652 mL | 1.9304 mL | 4.8260 mL | |
| 20 mM | 0.1448 mL | 0.7239 mL | 1.4478 mL | 3.6195 mL | |
| 25 mM | 0.1158 mL | 0.5791 mL | 1.1582 mL | 2.8956 mL | |
| 30 mM | 0.0965 mL | 0.4826 mL | 0.9652 mL | 2.4130 mL | |
| 40 mM | 0.0724 mL | 0.3620 mL | 0.7239 mL | 1.8098 mL | |
| 50 mM | 0.0579 mL | 0.2896 mL | 0.5791 mL | 1.4478 mL | |
| 60 mM | 0.0483 mL | 0.2413 mL | 0.4826 mL | 1.2065 mL | |
| 80 mM | 0.0362 mL | 0.1810 mL | 0.3620 mL | 0.9049 mL | |
| 100 mM | 0.0290 mL | 0.1448 mL | 0.2896 mL | 0.7239 mL |
Cusculine Related Classifications
HY-N17251 Related Classifications
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