Cy5-DBCO
Based on 4 publication(s) in Google Scholar
Cy5-DBCO (DBCO-Sulfo-Cy5) is a near-infrared (NIR)red fluorescent dye with λabsand λem of 646nm and 670 nm, respectively. Cy5-DBCO (DBCO-Sulfo-Cy5) is not suitable for staining intracellular components of permeabilezed cell, it may exhibits a high background. Cy5-DBCO is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
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- Pureté : 98.93%
- CAS No.: 1564286-24-3
- Formule: C52H56N4O11S3
- Masse moléculaire:1009.22
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Stockage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Cy5-DBCO
More
Activité biologique
Description
IC50 & Target
Red fluorescent dye
In Vitro
Cy5-DBCO (DBCO-Sulfo-Cy5) reacts with azides via a copper-free “click chemistry” reaction and forms a stable triazole and does not need Cu-catalyst or elevated temperatures.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Emission (Em)
668
Excitation (Ex)
647
Chemical Information
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CAS No. 1564286-24-3
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Appearance Solid
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Masse moléculaire 1009.22
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Formule C52H56N4O11S3
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Color Blue to black
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SMILES
O=S(CCC[N+]1=C(/C=C/C=C/C=C(N(CCCCCC(NCCC(N2C3=CC=CC=C3C#CC4=CC=CC=C4C2)=O)=O)C5=CC=C(C=C65)S(=O)(O)=O)/C6(C)C)C(C)(C)C7=CC(S(=O)(O)=O)=CC=C17)([O-])=O
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Synonyms
DBCO-Sulfo-Cy5
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (4)
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Journal Impact Factor
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Most Recent
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Nat Commun
Observation of structural switch in nascent SAM-VI riboswitch during transcription at single-nucleotide and single-molecule resolution. [Abstract]2023 Apr 22;14(1):2320. PMID: 37087479 -
Acta Biomater
Redox-responsive dendritic copolymer-drug conjugates enhance therapeutic mitophagy through coordinated microtubule destabilization for synergistic triple-negative breast cancer therapy. [Abstract]2025 Aug:202:248-261. PMID: 40555336 -
Cell Chem Biol
2026 Jun 16:S2451-9456(26)00196-0. PMID: 42302779 -
Solvant et solubilité
In Vitro:
DMSO : 62.5 mg/mL (61.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 10 mg/mL (9.91 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Pureté et documentation
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Fiche technique (255 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 0.9909 mL | 4.9543 mL | 9.9086 mL | 24.7716 mL |
| 5 mM | 0.1982 mL | 0.9909 mL | 1.9817 mL | 4.9543 mL | |
| DMSO | 10 mM | 0.0991 mL | 0.4954 mL | 0.9909 mL | 2.4772 mL |
| 15 mM | 0.0661 mL | 0.3303 mL | 0.6606 mL | 1.6514 mL | |
| 20 mM | 0.0495 mL | 0.2477 mL | 0.4954 mL | 1.2386 mL | |
| 25 mM | 0.0396 mL | 0.1982 mL | 0.3963 mL | 0.9909 mL | |
| 30 mM | 0.0330 mL | 0.1651 mL | 0.3303 mL | 0.8257 mL | |
| 40 mM | 0.0248 mL | 0.1239 mL | 0.2477 mL | 0.6193 mL | |
| 50 mM | 0.0198 mL | 0.0991 mL | 0.1982 mL | 0.4954 mL | |
| 60 mM | 0.0165 mL | 0.0826 mL | 0.1651 mL | 0.4129 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Cy5-DBCO Related Classifications
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