Art. -Nr.: HY-W753198

CY5 triethylamine

46 Cited Publications

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CY5 triethylamine is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.

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Formel: C₃₉H₅₅N₃O₈S₂
Molecular Weight:758.00

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Data Sheet (278 KB)

CY5 triethylamine Chemical Structure

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

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Chemical Information
Publications (46)
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Beschreibung

CY5 triethylamine is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis^[1]. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance^[2].

In Vitro

Protocol
1.Protein Preparetion
1) In order to obtain the best labeling effect, please prepare the protein (antibody) concentration as 2 mg/mL.
2) The pH value of protein solution shall be 8.5±0.5. If the pH is lower than 8.0, 1 M sodium bicarbonate shall be used for adjustment.
3) If the protein concentration is lower than 2 mg/mL, the labeling efficiency will be greatly reduced. In order to obtain the best labeling efficiency, it is recommended that the final protein concentration range is 2-10 mg/mL.
4) The protein must be in the buffer without primary amine (such as Tris or glycine) and ammonium ion, otherwise the labeling efficiency will be affected.
2.Dye Preparation (Example for CY3-NHS ester)
Add anhydrous DMSO into the vial of CY3-NHS ester to make a 10 mM stock solution. Mix well by pipetting or vortex.
Before use, it must be activated with condensation solution (500 μg/mL) (HY-D0178) before subsequent labeling experiments can be performed.
3.Calculation of dye dosage
The amount of CY3-NHS ester required for reaction depends on the amount of protein to be labeled, and the optimal molar ratio of CY3-NHS ester to protein is about 10.
Example: assuming the required marker protein is 500 μL 2 mg/mL IgG (MW=150,000), use 100 μL DMSO dissolve 1 mg CY3-NHS ester, the required CY3-NHS ester volume is 5.05 μL, and the detailed calculation process is as follows:
1) mmol (IgG) = mg/mL (IgG) ×mL (IgG) / MW (IgG) =2 mg/mL × 0.5 mL / 150,000 mg/mmol= 6.7×10-6 mmol
2) mmol (CY3-NHS ester) = mmol (IgG) × 10 = 6.7×10-6 mmol×10 = 6.7 × 10-5 mmol
3) μL (CY3-NHS ester) = mmol (CY3-NHS ester) ×MW (CY3-NHS ester) / mg/μL (CY3-NHS ester) = 6.7 ×10-5 mmol ×753.88 mg/mmol / 0.01 mg/μL = 5.05 μL (CY3-NHS ester)
4.Run conjugation reaction
1) A good volume of freshly prepared 10 mg/mL CY3-NHS ester is slowly added to 0.5 mL protein sample
In solution, gently shake to mix, then centrifuge briefly to collect the sample at the bottom of the reaction tube. Don'tmix well to prevent protein samples from denaturation and inactivation.
2) The reaction tubules were placed in a dark place and incubated gently at room temperature for 60 minutes at intervals.For 10-15 minutes, gently reverse the reaction tubules several times to fully mix the two reactants and raise the bar efficiency.
5.Purify the conjugation
The following protocol is an example of dye-protein conjugate purification by using a SepHadex G-25 column.
1) Prepare SepHadex G-25 column according to the manufacture instruction.
2) Load the reaction mixture (From "Run conjugation reaction") to the top of the SepHadex G-25 column.
3) Add PBS (pH 7.2-7.4) as soon as the sample runs just below the top resin surface.
4) Add more PBS (pH 7.2-7.4) to the desired sample to complete the column purification. Combine the fractions that contain the desired dye-protein conjugate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

Molecular Weight 758.00
Formel C₃₉H₅₅N₃O₈S₂
SMILES

CC(/C(N1CCCCCC(O)=O)=C\C=C\C=C\C2=[N+](CC)C(C=CC(S(=O)([O-])=O)=C3)=C3C2(C)C)(C)C4=C1C=CC(S(=O)(O)=O)=C4.CCN(CC)CC
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (46)

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Adv Funct Mater

2024 Jul 08.
ACS Nano

Self-Assembled Spatially Confined Mo-Based Nanoreactor for Multimechanistic Tumor Therapy Driven by Self-Cascade Catalysis. [Abstract]

2025 Dec 9;19(48):41368-41385. PMID: 41311256

CY5 triethylamine purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Dec 9;19(48):41368-41385. [Abstract]

Cy5-MTFGM (1-10 mg/mL; i.t.; i.v.). Ex vivo FL imaging of major tissues at different time points after injection with Cy5-labeled MTFGM.
ACS Nano

Biomimetic Nanoparticle-Mediated Target Delivery of Hypoxia-Responsive Plasmid of Angiotensin-Converting Enzyme 2 to Reverse Hypoxic Pulmonary Hypertension. [Abstract]

2023 May 9;17(9):8204-8222. PMID: 37071566

CY5 triethylamine purchased from MedChemExpress. Usage Cited in: ACS Nano. 2023 May 9;17(9):8204-8222. [Abstract]

Cy5-HTSFcACE2 plasmid (5 μg/mL; 1-6 h). Nuclear localization and lysosomal escape of ACE2-CS-PRT@PM in PMVECs in hypoxia for the indicated periods (red, HTSFcACE2 plasmid labeled with Cy5; green, lysosome labeled with Lysotracker; blue, DAPI to stain the nuclei).
J Nanobiotechnology

Renal-tubular-mitochondrial sequentially targeted nanoagent breaks the vicious cycle of oxidative stress and mtDNA-driven inflammation in acute kidney injury therapy. [Abstract]

2026 Jan 24;24(1):169. PMID: 41580683
J Nanobiotechnology

Programmable macrophage-polarizing nanoparticles for MRI-guided early detection and treatment of pulmonary metastases. [Abstract]

2025 Oct 14;23(1):675. PMID: 41084003

CY5 triethylamine purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Oct 14;23(1):675. [Abstract]

Cy5@CQM NPs (100 µL; C_Mn= 0.5 mM; intravenously injection). Immunofluorescence histology of lung tumors in mice at 30 min (left) and 2 h (right) p.i. of Cy5@CQM NPs. Dashed lines indicate tumor margins. Fluorescence labels: DAPI (blue), F4/80 (green), and Cy5@CQM NPs (red).

CY5 triethylamine purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Oct 14;23(1):675. [Abstract]

Cy5@CQM NPs (C_Mn= 0.1 mM; 1 h; 0.5-4 h). CLSM images of vesicle-mediated delivery from RAW264.7 cells to 4T1 cells, shown at various time points. Fluorescence labels: RAW264.7 cells and their extracellular vesicles (blue), 4T1 cells (green), and Cy5@CQM NPs (red). The white arrows indicate CQM NPs successfully delivered into 4T1 cells.
J Nanobiotechnology

Quadruple synergistic amplification of ferroptosis for precision glioblastoma therapy: a luteolin-coordinated ferric ion nanoplatform. [Abstract]

2025 Sep 25;23(1):605. PMID: 40999469
J Nanobiotechnology

5-FU@HFn combined with decitabine induces pyroptosis and enhances antitumor immunotherapy for chronic myeloid leukemia. [Abstract]

2025 Mar 28;23(1):252. PMID: 40148810
Adv Sci (Weinh)

Herba Lysimachiae Polysaccharide-Modified Selenium Nanoparticles Alleviate Oxidative Injury in Kidney Stones via TOMM22-Regulated Mitophagy Activation. [Abstract]

2026 May 19:e75784. PMID: 42154455
Adv Sci (Weinh)

BCMA-Engineered Dendritic Cell-Derived Exosomes as Bi-Functional Therapeutics Orchestrating Cytokine Sequestration and Immune Activation for Multiple Myeloma. [Abstract]

2026 May 15:e75686. PMID: 42138792
Adv Sci (Weinh)

All-In-One OsciDrop Digital PCR System for Automated and Highly Multiplexed Molecular Diagnostics. [Abstract]

2024 Jun;11(21):e2309557. PMID: 38516754
Sci Adv

A softness zwitterionic micelles efficiently deliver inhaled nintedanib by enhancing airway mucus penetration. [Abstract]

2026 Jan 2;12(1):eady1030. PMID: 41481743
Biomaterials

Reversing neuroinflammation in ischemic stroke through efferocytotic phenotype reprogramming with polymeric nanoparticles. [Abstract]

2026 Sep:332:124156. PMID: 41895020
Asian J Pharm Sci

Photothermal-detonated functional macrophage membrane-camouflaged nano-crackers induce tumor cell wandering-anoikis. [Abstract]

2026 Jun;21(3):101171. PMID: 42338758
Chem Eng J

2025 Oct 20.
Chem Eng J

2025 Jan 7.

CY5 triethylamine purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Jan 7.

Fluorescence of bone marrow from mice sacrificed 24 h after intravenous injection of PBS, equivalent Cy5 dissolved in PBS, Cy5 labeled exosomes.
Small

Lung Tissue-Targeting STING mRNA-LNPs Inhibits Tumor Progression and Induces Systemic Anti-Tumor Immunity in Non-Small Cell Lung Cancer. [Abstract]

2025 Sep 15:e04715. PMID: 40948207
Phytomedicine

Capsaicin ameliorates cholestasis through modulation of the FXR-SHP and FXR-FGF15 gut-liver axis in mice. [Abstract]

2025 Oct:146:157130. PMID: 40782765
Mater Today Bio

A tumor microenvironment-responsive Zr-MOF nanosystem for co-delivering siHIF-1α and triptolide enhances photodynamic therapy in esophageal cancer by amplifying ROS generation and reversing hypoxia. [Abstract]

2025 Aug 13:34:102183. PMID: 40893379
Adv Healthc Mater

Engineered Hollow Nanocomplex Combining Photothermal and Antioxidant Strategies for Targeted Tregs Depletion and Potent Immune Activation in Tumor Immunotherapy. [Abstract]

2025 Jun;14(15):e2405124. PMID: 40109122
Mater Today Bio

GlycoRNA-rich, neutrophil membrane-coated, siMT1-loaded nanoparticles mitigate abdominal aortic aneurysm progression by inhibiting the formation of neutrophil extracellular traps. [Abstract]

2025 Mar 4:31:101630. PMID: 40124343
Nano Today

2024 Dec.
Acta Biomater

Decellularized amnion membrane-based thermosensitive vaginal hydrogel enhances ritodrine efficacy and reduces systemic side effects in preterm birth treatment. [Abstract]

2025 Jul 1:201:212-228. PMID: 40516841
Food Res Int

2025 Dec 28;227:118191.
Int J Nanomedicine

ROS-pH Dual-Responsive Polydopamine Nanoparticles for Targeted Fasudil Delivery Ameliorate Multiple Pathologies in Diabetic Retinopathy. [Abstract]

2026 May 27;21. PMID: 42232487
Int J Biol Macromol

Hyaluronic acid-modified PtPdCo-CQ nanocatalyst with triple enzyme-like activities regulates macrophage polarization and autophagy levels for the treatment of rheumatoid arthritis. [Abstract]

2025 Apr 13;309(Pt 4):143143. PMID: 40233904
Int J Nanomedicine

Netrin-1 Monoclonal Antibody-Functionalized Nanoparticle Loaded with Metformin Prevents the Progression of Abdominal Aortic Aneurysms. [Abstract]

2023 Feb 6:18:627-639. PMID: 36777816
ACS Appl Mater Interfaces

Regulation of Bone Remodeling by Metal-Phenolic Networks for the Treatment of Systemic Osteoporosis. [Abstract]

2025 Jan 29;17(4):5995-6008. PMID: 39818714
ACS Appl Mater Interfaces

Affimer Tagged Cubosomes: Targeting of Carcinoembryonic Antigen Expressing Colorectal Cancer Cells Using In Vitro and In Vivo Models. [Abstract]

2022 Mar 9;14(9):11078-11091. PMID: 35196008
Br J Pharmacol

Reprogramming tumour-associated macrophages in breast cancer via si-FOXM1-loaded lipid nanoparticles enhances immune checkpoint inhibitor efficacy. [Abstract]

2026 Jun;183(11):3049-3073. PMID: 41723880
ACS Biomater Sci Eng

Tailorable Metal-Polyphenol Nanoparticles for Targeted Treatment of Inflammatory Bowel Disease. [Abstract]

2026 Mar 9;12(3):1616-1632. PMID: 41732806
Colloids Surf B Biointerfaces

ROS responsive hydrogel for inhibition of MUC5AC against allergic rhinitis: A new delivery strategy for Ipratropium Bromide. [Abstract]

2024 Oct:242:114112. PMID: 39047643
ACS Appl Nano Mater

2026 Jan 9;9(3):1696-1709.
Int Immunopharmacol

Calcitonin gene-related peptide-modulated macrophage phenotypic alteration regulates angiogenesis in early bone healing. [Abstract]

2024 Mar 30:130:111766. PMID: 38452411
Eur J Pharm Sci

Monitoring endosomal escape of fluorescent cell-penetrating peptides. [Abstract]

2025 Dec 1:215:107330. PMID: 41101513
Eur J Pharm Sci

Uterine first-pass effect: Unlocking the potential of vaginally administered ritodrine-loaded thermosensitive gel for uterine drug delivery. [Abstract]

2025 Jan 1:204:106945. PMID: 39489073
Eur J Pharm Biopharm

Peptide-based hydrogel co-assembled with antibody-drug for enhanced retinal cell uptake and attenuated experimental autoimmune uveitis. [Abstract]

2025 May:210:114691. PMID: 40054506
Pest Manag Sci

Selenium nanoparticles are effective in penetrating pine and causing high oxidative damage to Bursaphelenchus xylophilus in pine wilt disease control. [Abstract]

2022 Aug;78(8):3704-3716. PMID: 35643940
J Biomed Mater Res A

Hyaluronic Acid-Chitosan Nanoparticles Encapsulating Gal-9 Alleviate Severe Acute Pancreatitis by Promoting M2 Macrophage Polarization. [Abstract]

2025 Dec;113(12):e70006. PMID: 41307175
Metab Brain Dis

ROS-responsive dextran-benzeneboronic acid pinacol ester micelles encapsulating edaravone for the treatment and mechanism of cerebral ischemia-reperfusion injury. [Abstract]

2025 May 13;40(5):202. PMID: 40358747
Mol Biol Rep

Yam glycoprotein mediated the immunomodulation of macrophages by targeting TLR4. [Abstract]

2026 Jun 18;53(1):954. PMID: 42313284
PLoS One

Smart thermosensitive poloxamer hydrogels loaded with Nr-CWs for the treatment of diabetic wounds. [Abstract]

2022 Dec 30;17(12):e0279727. PMID: 36584197
chemRxiv
bioRxiv

2026 Jan 30.
University of California

2025.
Int J Radiat Biol

Bovine serum albumin and folic acid-modified aurum nanoparticles loaded with paclitaxel and curcumin enhance radiotherapy sensitization for esophageal cancer. [Abstract]

2024;100(3):411-419. PMID: 37934908
Research Square Preprint

2021 Jul.

Reinheit & Dokumentation

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Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Ptaszek M. Rational design of fluorophores for in vivo applications. Prog Mol Biol Transl Sci. 2013;113:59-108. [Content Brief]

[2]. Shindy, H. A. (2017). Fundamentals in the chemistry of cyanine dyes: A review. Dyes and Pigments, 145, 505–513. doi:10.1016/j.dyepig.2017.06.029

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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CY5 triethylamine

46 Cited Publications

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CY5 triethylamine

Publications Citing Use of MedChemExpress (MCE) CY5 triethylamine

Biologische Aktivität

Chemical Information

Publications (46)

Reinheit & Dokumentation

Verweise

Calculators

Help & FAQs

CY5 triethylamine Related Classifications

HY-W753198 Related Classifications