Cy5-ZW2-617
Cy5-ZW2-617 is a PSMA ligand with a IC50 of 4.39 nM. Cy5-ZW2-617 specifically binds to PSMA and is thereby internalized by PSMA-positive cells. Cy5-ZW2-617 shows uptake in PSMA-positive tumor cells and tumor xenografts in mice. Cy5-ZW2-617 can be used for the research of prostate cancer.
For research use only. We do not sell to patients.
- Formula: C89H123N17O24S4
- Molecular Weight:1943.29
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Cy5-ZW2-617 (Compound 10) (2.5-10 μM; 2 h) is efficiently and specifically endocytosed by PSMA-positive PC3-PIP and LNCaP cells, and then mainly localizes to the perinuclear compartment, while no uptake is observed in PSMA-negative PC3 flu cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:PSMA-positive PC3-PIP, LNCaP cells
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Concentration:2.5 μM or 10 μM
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Incubation Time:2 h
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Result:Showed efficient internalization into PSMA-positive PC3-PIP and LNCaP cells, with highest fluorescence intensity observed in perinuclear compartments.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:athymic nude-Fox1nu (male, 6 weeks old, subcutaneous xenograft modeling with PSMA-positive PC3-PIP and PSMA-negative PC3 flu cells)[1]
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Dosage:20 nmol
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Administration:retro-orbital; single dose
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Result:Exhibited high, specific uptake in PSMA-positive PC3-PIP tumors, with low uptake in PSMA-negative PC3 flu tumors.
Reduced uptake in head and neck region 28-fold compared to reference compound Cy5-617 8.
Improved tumor-to-muscle ratio 4-fold compared to reference compound Cy5-617 8.
Improved tumor-to-head and neck region ratio 12-fold compared to reference compound Cy5-617 8.
Chemical Information
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Molecular Weight 1943.29
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Formula C89H123N17O24S4
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SMILES
C[N+](CCCS(=O)([O-])=O)(C)CC(N=N1)=CN1CC[C@H](NC([C@H](CCN2C=C(N=N2)C[N+](CCCS(=O)([O-])=O)(C)C)NC(CCCCCN(/C3=C/C=C/C=C/C(C4(C)C)=[N+](C5=C4C=C(S(=O)([O-])=O)C=C5)C)C6=C(C=C(S(=O)(O)=O)C=C6)C3(C)C)=O)=O)C(NC[C@H]7CC[C@@H](CC7)C(N[C@@H](CC8=CC(C=CC=C9)=C9C=C8)C(NCCCC[C@H](NC(N[C@@H](CCC(O)=O)C(O)=O)=O)C(O)=O)=O)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Cy5-ZW2-617 Related Classifications
HY-182240 Related Classifications
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