Cyanine7.5 amine

Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
1. Preparation of Reagents:
(1) Dye Solution: Dissolve Cyanine7.5 amine in dry DMF or DMSO to create a 10-50 mg/mL stock solution.
(2) Biomolecule Solution: Dissolve the protein/antibody in a coupling buffer (e.g., 50 mM borate buffer or PBS, pH 8.3-8.5) at a concentration of 1-10 mg/mL.
2. Reaction Procedure:
(1) Slowly add the Cyanine7.5 amine stock solution into the protein solution while stirring. Use a molar excess of dye (typically 5-20 fold relative to the protein).
(2) Incubate the mixture at room temperature for 1-2 hours or at 4°C overnight, protected from light.
3. Purification:
Remove unreacted dye using gel filtration (e.g., Sephadex G-25) or dialysis against buffer.
Considerations
1. Solubility: Standard Cyanine7.5 amine is hydrophobic; ensure enough co-solvent (DMSO/DMF) is used.
2. pH: The amine group must be deprotonated to react, making a slightly basic pH (8.3-8.5) ideal.
3. Applications: Ideal for near-infrared imaging (788 nm excitation/808 nm emission) of live animals.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.