Cyanine7.5 amine 

Cyanine7.5 amine is a near-infrared Cy7.5-based dye and also a substrate for amide bond coupling (Ex/Em = 788 nm/808 nm). Cyanine7.5 amine contains a free amino group and can be used for coupling with activated carboxylic acid derivatives^[1].

Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
1. Preparation of Reagents:
(1) Dye Solution: Dissolve Cyanine7.5 amine in dry DMF or DMSO to create a 10-50 mg/mL stock solution.
(2) Biomolecule Solution: Dissolve the protein/antibody in a coupling buffer (e.g., 50 mM borate buffer or PBS, pH 8.3-8.5) at a concentration of 1-10 mg/mL.
2. Reaction Procedure:
(1) Slowly add the Cyanine7.5 amine stock solution into the protein solution while stirring. Use a molar excess of dye (typically 5-20 fold relative to the protein).
(2) Incubate the mixture at room temperature for 1-2 hours or at 4°C overnight, protected from light.
3. Purification:
Remove unreacted dye using gel filtration (e.g., Sephadex G-25) or dialysis against buffer.
Considerations
1. Solubility: Standard Cyanine7.5 amine is hydrophobic; ensure enough co-solvent (DMSO/DMF) is used.
2. pH: The amine group must be deprotonated to react, making a slightly basic pH (8.3-8.5) ideal.
3. Applications: Ideal for near-infrared imaging (788 nm excitation/808 nm emission) of live animals.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

819.99

C₅₁H₆₄Cl₂N₄O

2104005-17-4

Solid

Green to dark green

O=C(CCCCCN1C2=C(C(C)(C)/C1=C\C=C3C=C(CCC\3)/C=C/C4=[N+](C)C5=C(C4(C)C)C6=C(C=CC=C6)C=C5)C7=C(C=CC=C7)C=C2)NCCCCCCN.[Cl-].Cl

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

• [1]. Retout M, et al. Bifunctional Calix[4]arene-Coated Gold Nanoparticles for Orthogonal Conjugation. Langmuir. 2022;38(30):9301-9309.