2. Autophagy GPCR/G Protein
  3. Autophagy ICMT
  4. Cysmethynil

Cysmethynil is an Icmt inhibitor(IC50 = 2.4 μM). Cysmethynil inhibites RAS membrane binding and EGF signal transduction. Cysmethynil prevents the cells in the G1 phase and induces autophagy. Cysmethynil inhibits PC3 cells proliferation, has synergistic effect with Paclitaxel (HY-B0015) and Doxorubicin (HY-15142A). Cysmethynil has anti-tumor effects and can be used for solid tumor (such as prostate cancer et al.) research.

For research use only. We do not sell to patients.

Cysmethynil Chemical Structure

Cysmethynil Chemical Structure

CAS No. : 851636-83-4

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Based on 1 publication(s) in Google Scholar

Cysmethynil Related Antibodies

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Description

Cysmethynil is an Icmt inhibitor(IC50 = 2.4 μM). Cysmethynil inhibites RAS membrane binding and EGF signal transduction. Cysmethynil prevents the cells in the G1 phase and induces autophagy. Cysmethynil inhibits PC3 cells proliferation, has synergistic effect with Paclitaxel (HY-B0015) and Doxorubicin (HY-15142A). Cysmethynil has anti-tumor effects and can be used for solid tumor (such as prostate cancer et al.) research[1][2][3].

IC50 & Target

IC50 = 2.4 μM for Icmt
In Vitro

Cysmethynil (15 μM or 20 μM or 30 μM; 6 days) inhibits Icmt+/+ cells and Icmt-/-/ICMT cells proliferation, unaffects Icmt-/-cells growth [1].
Cysmethynil (20-30 μM; 1-6 days) inhibits PC3 cells proliferation and results in a dose- and time-dependent reduction in the number of viable PC3 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cysmethynil Related Antibodies

Cell Viability Assay

Cell Line: PC3 cells[2]
Concentration: 20-30 μM cysmethynil
Incubation Time: 1-6 days
Result: Resulted in a dose- and time-dependent reduction in the number of viable PC3 cells.
In Vivo

Cysmethynil (100 mg/kg, 200 mg/kg; intraperitoneal injection; every 48 h; 28 day) without adverse effects on mice body weight, significantly impacts on tumor growth, causes both an accumulation of cells in the G1 phase and cell death [2].
Cysmethynil (20 mg/kg; intraperitoneal injection; three times a week; 2 weeks; alone or combined with Paclitaxel  (HY-B0015)/ Doxorubicin (HY-15142A) ) is not toxic to mice and induces only moderate inhibition of tumor growth as single agent, the combination with Paclitaxel(HY-B0015)/ Doxorubicin(HY-15142A) results in significantly greater efficacy in inhibiting tumor growth. Cysmethynil sensitizes cervical cancer cells to chemotherapy agent in xenograft mouse model[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 6 week-old SCID mice with SiHa cells in the mice flank [3]
Dosage: 20 mg/kg (single; combination with Paclitaxel or Doxorubicin)
Administration: intraperitoneal injection; three times a week; 2 weeks
Result: Decreased in tumor size when treated with Cys alone, or Cysmethynil+Paclitaxel(HY-B0015)/Cysmethynil+ Doxorubicin(HY-15142A) .
Animal Model: SCID mice with PC3 cells in a xenograft model[2]
Dosage: 0.1 mg/g or 0.2 mg/g Cysmethynil
Administration: intraperitoneal injection; every 48 h; 28 days
Result: Decreased in tumor size when treated with both doses of cysmethynil.
Molecular Weight

376.53

Formula

C25H32N2O
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(N)CC1=CN(CCCCCCCC)C2=C1C=C(C3=CC=CC(C)=C3)C=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (265.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6558 mL 13.2792 mL 26.5583 mL
5 mM 0.5312 mL 2.6558 mL 5.3117 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6558 mL 13.2792 mL 26.5583 mL 66.3958 mL
5 mM 0.5312 mL 2.6558 mL 5.3117 mL 13.2792 mL
10 mM 0.2656 mL 1.3279 mL 2.6558 mL 6.6396 mL
15 mM 0.1771 mL 0.8853 mL 1.7706 mL 4.4264 mL
20 mM 0.1328 mL 0.6640 mL 1.3279 mL 3.3198 mL
25 mM 0.1062 mL 0.5312 mL 1.0623 mL 2.6558 mL
30 mM 0.0885 mL 0.4426 mL 0.8853 mL 2.2132 mL
40 mM 0.0664 mL 0.3320 mL 0.6640 mL 1.6599 mL
50 mM 0.0531 mL 0.2656 mL 0.5312 mL 1.3279 mL
60 mM 0.0443 mL 0.2213 mL 0.4426 mL 1.1066 mL
80 mM 0.0332 mL 0.1660 mL 0.3320 mL 0.8299 mL
100 mM 0.0266 mL 0.1328 mL 0.2656 mL 0.6640 mL
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Cysmethynil Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cysmethynil851636-83-4AutophagyICMTIsoprenylcysteine carboxyl methyltransferasePC3 cellsMouse embryonic fibroblastsxenograft modelSiHa cellsInhibitorinhibitorinhibit

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